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Literature summary extracted from
- Enhancing the thermostability of alpha-glucosidase from Thermoanaerobacter tengcongensis MB4 by single proline substitution (2010), J. Biosci. Bioeng., 110, 12-17.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | gene gluA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Caldanaerobacter subterraneus subsp. tengcongensis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | K285P | site-directed mutagenesis, the mutant shows increased thermostability but no distinct changes in its secondary structure compared to the wild-type enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
| 3.2.1.20 | L152P | site-directed mutagenesis, the mutant shows increased thermostability but no distinct changes in its secondary structure compared to the wild-type enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
| 3.2.1.20 | N208P | site-directed mutagenesis, the mutant shows no increased thermostability compared to the wild-type enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
| 3.2.1.20 | T430P | site-directed mutagenesis, the mutant shows increased thermostability but no distinct changes in its secondary structure compared to the wild-type enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 0.32 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant K285P | Caldanaerobacter subterraneus subsp. tengcongensis |  |
| 3.2.1.20 | 1.08 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, wild-type enzyme | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 3.2.1.20 | 1.61 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant L152P | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 3.2.1.20 | 1.64 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant T430P | Caldanaerobacter subterraneus subsp. tengcongensis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Caldanaerobacter subterraneus subsp. tengcongensis | Q8RDL1 | gene gluA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography and gel filtration | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | More | secondary structure of wild-type and mutant enzymes and three-dimensional structure modeling, overview | Caldanaerobacter subterraneus subsp. tengcongensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | GluA | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 60 | - |
assay at | Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 74 | - |
recombinant mutant N208P shows 20% remaining activity, while the wild-type enzyme shows 32% residual activity | Caldanaerobacter subterraneus subsp. tengcongensis |
| 3.2.1.20 | 77 | - |
15 min, the recombinant wild-type is almost inactive, while mutants K285P, L152P, and T430P retain 67%, 25%, and 20% residual activity, respectively | Caldanaerobacter subterraneus subsp. tengcongensis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 1.72 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant K285P | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 3.2.1.20 | 4.27 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant L152P | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 3.2.1.20 | 6.64 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, wild-type enzyme | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 3.2.1.20 | 7.25 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant T430P | Caldanaerobacter subterraneus subsp. tengcongensis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 5.5 | - |
assay at | Caldanaerobacter subterraneus subsp. tengcongensis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | additional information | Leu152, Lys285, and Thr430 are not only important for the thermostability but also related to the substrate binding and catalytic activity of TtGluA | Caldanaerobacter subterraneus subsp. tengcongensis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 2.65 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant L152P | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 3.2.1.20 | 4.42 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant T430P | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 3.2.1.20 | 5.37 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, mutant K285P | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 3.2.1.20 | 6.15 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 60°C, wild-type enzyme | Caldanaerobacter subterraneus subsp. tengcongensis | |
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