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Literature summary extracted from

  • Fan, C.; Fromm, H.J.; Bobik, T.A.
    Kinetic and functional analysis of L-threonine kinase, the PduX enzyme of Salmonella enterica (2009), J. Biol. Chem., 284, 20240-20248.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.177 D103N similar growth rate compared to wild-type Salmonella enterica
2.7.1.177 D135A no activity, decrease in helical structure Salmonella enterica
2.7.1.177 D135N similar growth rate compared to wild-type Salmonella enterica
2.7.1.177 E132A similar growth rate compared to wild-type Salmonella enterica
2.7.1.177 E132Q similar growth rate compared to wild-type Salmonella enterica
2.7.1.177 E24A no activity, decrease in helical structure Salmonella enterica
2.7.1.177 E24Q similar growth rate compared to wild-type Salmonella enterica
2.7.1.177 S100A no activity Salmonella enterica
2.7.1.177 S253A low growth rate, reduced affinity for L-threonine Salmonella enterica
2.7.1.177 S255A low growth rate, reduced affinity for L-threonine Salmonella enterica
2.7.1.177 S99A similar growth rate compared to wild-type Salmonella enterica
2.7.1.177 T101A similar growth rate compared to wild-type Salmonella enterica
2.7.1.177 T134A similar growth rate compared to wild-type Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.177 0.0547
-
 ATP wild-type, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.068
-
 ATP mutant D135N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.074
-
 ATP mutant S255A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.076
-
 ATP mutant S253A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.087
-
 ATP mutant T134A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.098
-
 ATP mutant T101A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.117
-
 ATP mutant E24Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.141
-
 ATP mutant D103N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.142
-
 L-threonine mutant T134A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.144
-
 L-threonine mutant E132Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.146
-
 L-threonine wild-type, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.163
-
 ATP mutant D103A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.176
-
 L-threonine mutant S99A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.184
-
 L-threonine mutant D103N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.198
-
 L-threonine mutant E132A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.22
-
 L-threonine mutant T101A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.258
-
 L-threonine mutant D103A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.273
-
 L-threonine mutant D135N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.464
-
 L-threonine mutant E24Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 1.264
-
 ATP mutant E132Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 1.374
-
 ATP mutant S99A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 1.979
-
 ATP mutant E132A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 3.708
-
 L-threonine mutant S255A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 4.698
-
 L-threonine mutant S253A, pH 7.4, temperature not specified in the publication Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.177 35000
-
 x * 35000, SDS-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.177 ATP + L-threonine Salmonella enterica
-
 ADP + O-phospho-L-threonine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.177 Salmonella enterica Q9XDM4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.177 nickel-nitrilotriacetate column Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.177 ATP + L-threonine
-
 Salmonella enterica ADP + O-phospho-L-threonine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.177 ? x * 35000, SDS-PAGE Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
2.7.1.177 PduX
-
 Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.177 0.016
-
 L-threonine mutant D135N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0173
-
 ATP mutant D135N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0187
-
 ATP mutant E24Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0188
-
 L-threonine mutant E24Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0193
-
 L-threonine mutant E132A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0205
-
 ATP mutant E132A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0227
-
 ATP mutant S255A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0238
-
 L-threonine mutant S255A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0258
-
 L-threonine mutant E132Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.026
-
 ATP mutant S253A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0272
-
 ATP mutant E132Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0287
-
 L-threonine mutant S253A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0313
-
 ATP mutant D103A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0322
-
 L-threonine mutant D103A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0325
-
 L-threonine mutant S99A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0338
-
 ATP mutant D103N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0338
-
 ATP mutant S99A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0342
-
 ATP mutant T134A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0353
-
 L-threonine mutant T134A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0355
-
 ATP mutant T101A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0358
-
 L-threonine mutant D103N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.037
-
 L-threonine mutant T101A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0427
-
 L-threonine wild-type, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.408
-
 ATP wild-type, pH 7.4, temperature not specified in the publication Salmonella enterica

General Information

EC Number General Information Comment Organism
2.7.1.177 metabolism involved in the biosynthesis of adenosylcobalamin Salmonella enterica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.177 0.000061
-
 L-threonine mutant S253A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0000643
-
 L-threonine mutant S255A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.000104
-
 ATP mutant E132A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00022
-
 ATP mutant E132Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.000247
-
 ATP mutant S99A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.000407
-
 L-threonine mutant E24Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.000587
-
 L-threonine mutant D135N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00098
-
 L-threonine mutant E132A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00125
-
 L-threonine mutant D103A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00159
-
 ATP mutant E24Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00168
-
 L-threonine mutant T101A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0018
-
 L-threonine mutant E132Q, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00185
-
 L-threonine mutant S99A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00193
-
 ATP mutant D103A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00195
-
 L-threonine mutant D103N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.0024
-
 ATP mutant D103N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00248
-
 L-threonine mutant T134A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00257
-
 ATP mutant D135N, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00292
-
 L-threonine wild-type, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00308
-
 ATP mutant S255A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00342
-
 ATP mutant S253A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00363
-
 ATP mutant T101A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00393
-
 ATP mutant T134A, pH 7.4, temperature not specified in the publication Salmonella enterica
2.7.1.177 0.00755
-
 ATP wild-type, pH 7.4, temperature not specified in the publication Salmonella enterica