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Literature summary extracted from

  • Kotake, T.; Hirata, N.; Degi, Y.; Ishiguro, M.; Kitazawa, K.; Takata, R.; Ichinose, H.; Kaneko, S.; Igarashi, K.; Samejima, M.; Tsumuraya, Y.
    Endo-beta-1,3-galactanase from winter mushroom Flammulina velutipes (2011), J. Biol. Chem., 286, 27848-27854.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.181 expressed in Pichia pastoris Flammulina velutipes

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.181 E138D no activity Flammulina velutipes
3.2.1.181 E143D no activity Flammulina velutipes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.181 additional information
-
 beta-1,3-galactan the value for beta-1,3-galactan is 0.137 mg/ml Flammulina velutipes
3.2.1.181 0.57
-
 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes
3.2.1.181 1.25
-
 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.181 26690
-
 calculated from amino acid sequence Flammulina velutipes
3.2.1.181 30000
-
 SDS-PAGE Flammulina velutipes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.181 Flammulina velutipes F7J1C8 winter mushroom
-
3.2.1.181 Flammulina velutipes NBRC 7663 F7J1C8 winter mushroom
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.181 glycoprotein
-
 Flammulina velutipes

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.181
-
 Flammulina velutipes

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.181 The enzyme specifically hydrolyses beta-1,3-galactan and beta-1,3-galactooligosaccharides beta-1,4- and beta-1,6-galactooligosaccharides, agarose, or keratan sulfate do not serve as substrates Flammulina velutipes
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.181 mycelium
-
 Flammulina velutipes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.181 12.8
-
 pH 5.0, 37°C, liberation of D-galactose Flammulina velutipes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.181 beta-1,3-galactan + H2O
-
 Flammulina velutipes oligo-beta-(1->3)-D-galactose
-
 ?
3.2.1.181 beta-1,3-galactan + H2O
-
 Flammulina velutipes NBRC 7663 oligo-beta-(1->3)-D-galactose
-
 ?
3.2.1.181 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
 Flammulina velutipes 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
 ?
3.2.1.181 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
 Flammulina velutipes NBRC 7663 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
 ?
3.2.1.181 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
 Flammulina velutipes 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
 ?
3.2.1.181 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
 Flammulina velutipes NBRC 7663 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
 ?
3.2.1.181 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
 Flammulina velutipes 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
 ?
3.2.1.181 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
 Flammulina velutipes NBRC 7663 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose
-
 ?
3.2.1.181 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O
-
 Flammulina velutipes 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + D-galactopyranose after 30 min of reaction time the enzyme produces 42% D-glalactose, 30% dimeric, 20% trimeric and less than 10% tetrameric or pentameric beta-1,3-galactooligosaccharides ?
3.2.1.181 additional information The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity. Flammulina velutipes ?
-
 ?
3.2.1.181 additional information The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity. Flammulina velutipes NBRC 7663 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.181 endo-beta-1,3-galactanase
-
 Flammulina velutipes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.181 45
-
 recombinant enzyme Flammulina velutipes
3.2.1.181 50
-
 native enzyme Flammulina velutipes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.181 5.47
-
 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes
3.2.1.181 6.5
-
 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.181 4 6
-
 Flammulina velutipes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.181 1.14
-
 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes
3.2.1.181 4.38
-
 beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose pH 5.0, 37°C Flammulina velutipes