BRENDA - Enzyme Database

Isopeptide ligation catalyzed by quintessential sortase A: mechanistic cues from cyclic and branched oligomers of indolicidin

Dasgupta, S.; Samantaray, S.; Sahal, D.; Roy, R.P.; J. Biol. Chem. 286, 23996-24006 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.4.22.70
expressed in Escherichia coli BL21(DE3) cells
Staphylococcus aureus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.22.70
1.18
-
agmatine
at 37°C, pH not specified in the publication, transpeptidation reaction
Staphylococcus aureus
3.4.22.70
1.59
-
YALPETGK
at 37°C, pH not specified in the publication
Staphylococcus aureus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.22.70
Staphylococcus aureus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.4.22.70
Ni-NTA column chromatography
Staphylococcus aureus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.22.70
agmatine + YALPETGK
transpeptidase reaction. The initially formed acyl-enzyme intermediate undergoes a hydrolysis followed by intra-molecular transpeptidation
717831
Staphylococcus aureus
(NH2)2-CN-(CH2)4-NH-(CO)-TEPLAY + ?
-
-
-
?
3.4.22.70
additional information
sortase A also mediates transpeptidation reaction of indolicidin-derived peptides
717831
Staphylococcus aureus
?
-
-
-
-
3.4.22.70
YALPETGK + H2O
-
717831
Staphylococcus aureus
YALPET + Gly-Lys
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.22.70
expressed in Escherichia coli BL21(DE3) cells
Staphylococcus aureus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.22.70
1.18
-
agmatine
at 37°C, pH not specified in the publication, transpeptidation reaction
Staphylococcus aureus
3.4.22.70
1.59
-
YALPETGK
at 37°C, pH not specified in the publication
Staphylococcus aureus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.22.70
Ni-NTA column chromatography
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.22.70
agmatine + YALPETGK
transpeptidase reaction. The initially formed acyl-enzyme intermediate undergoes a hydrolysis followed by intra-molecular transpeptidation
717831
Staphylococcus aureus
(NH2)2-CN-(CH2)4-NH-(CO)-TEPLAY + ?
-
-
-
?
3.4.22.70
additional information
sortase A also mediates transpeptidation reaction of indolicidin-derived peptides
717831
Staphylococcus aureus
?
-
-
-
-
3.4.22.70
YALPETGK + H2O
-
717831
Staphylococcus aureus
YALPET + Gly-Lys
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
3.4.22.70
physiological function
the enzyme catalyzes the covalent anchoring of surface proteins to the cell wall by linking the threonyl carboxylate of the LPXTG recognition motif to the amino group of the pentaglycine cross-bridge of the peptidoglycan
Staphylococcus aureus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.4.22.70
physiological function
the enzyme catalyzes the covalent anchoring of surface proteins to the cell wall by linking the threonyl carboxylate of the LPXTG recognition motif to the amino group of the pentaglycine cross-bridge of the peptidoglycan
Staphylococcus aureus