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Literature summary extracted from

  • McLuskey, K.; Paterson, N.G.; Bland, N.D.; Isaacs, N.W.; Mottram, J.C.
    Crystal structure of Leishmania major oligopeptidase B gives insight into the enzymatic properties of a trypanosomatid virulence factor (2010), J. Biol. Chem., 285, 39249-39259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.83 expression in Escherichia coli Leishmania major

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.83 in complex with the inhibitor antipain and as mercury derivative, to 1.65 and 2.0 A resolution, respectively. OPB comprises two domains, a catalytic and a propeller domain, linked together by a hinge region. Residue E621 plays a critical role in the S1 binding pocket and, along with F603, is largely responsible for the enzyme substrate specificity in P1. In the S2 binding pocket, Y499 is important for substrate stability. Residue E623 forms an inter-domain hydrogen bond Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 antipain
-
 Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.83 0.00093
-
 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 0.0012
-
 benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 0.0031
-
 N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 0.0038
-
 N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 0.0044
-
 benzoyl-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Leishmania major Q4QHU7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 benzoyl-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Leishmania major benzoyl-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Leishmania major benzyloxycarbonyl-Gly-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 additional information no substrate: N-benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin Leishmania major ?
-
 ?
3.4.21.83 N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Leishmania major N-benzyloxycarbonyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Leishmania major N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Leishmania major N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.83 0.01
-
 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 0.011
-
 N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 0.013
-
 benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 0.016
-
 benzoyl-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 0.02
-
 N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.83 1
-
 benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 1.1
-
 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 3.6
-
 benzoyl-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 3.6
-
 N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major
3.4.21.83 5.3
-
 N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin 21°C, pH not specified in the publication Leishmania major