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Literature summary extracted from

  • McLean, K.; Lafite, P.; Levy, C.; Cheesman, M.; Mast, N.; Pikuleva, I.; Leys, D.; Munro, A.
    The structure of Mycobacterium tuberculosis CYP125: Molecular basis for cholesterol binding in a P450 needed for host infection (2009), J. Biol. Chem., 284, 35524-35533.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.29 expressed in Escherichia coli HMS174 (DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.29 sitting drop vapor diffusion method, using MgCl2 with 0.1 M HEPES, pH 7.0 or 7.5, and 20% (w/v) PEG 6000 or 25% (w/v) PEG 3350, respectively Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.29 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.29 Ni-NTA resin column chromatography, Resource-Q column chromatography, and Sephacryl S-200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.29 cholesterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycobacterium tuberculosis 27-hydroxycholesterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.29 CYP125
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.29 heme
-
 Mycobacterium tuberculosis
1.14.15.29 NADPH
-
 Mycobacterium tuberculosis