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Literature summary extracted from

  • Zhang, Y.; Teoh, K.H.; Reed, D.W.; Maes, L.; Goossens, A.; Olson, D.J.; Ross, A.R.; Covello, P.S.
    The molecular cloning of artemisinic aldehyde DELTA11(13) reductase and its role in glandular trichome-dependent biosynthesis of artemisinin in Artemisia annua (2008), J. Biol. Chem., 283, 21501-21508.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.92 expression in Saccharomyces cerevisiae Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.92 0.019
-
 artemisinic aldehyde 30°C, pH 7.5 Artemisia annua
1.3.1.92 0.095
-
 NADPH 30°C, pH 7.5 Artemisia annua
1.3.1.92 0.65
-
 (+)-carvone 30°C, pH 7.5 Artemisia annua
1.3.1.92 0.77
-
 NADH 30°C, pH 7.5 Artemisia annua
1.3.1.92 0.79
-
 2-cyclohexen-1-one 30°C, pH 7.5 Artemisia annua

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.92 cytosol
-
 Artemisia annua 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.92 44000
-
 gel filtration Artemisia annua

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.92 Artemisia annua C5H429
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.92 partial Artemisia annua

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.92 flower flower bud, moderate expression Artemisia annua
-
1.3.1.92 leaf moderate expression Artemisia annua
-
1.3.1.92 trichome glandular trichome, high expression Artemisia annua
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.92 (+)-carvone + NADH + H+
-
 Artemisia annua (1R,4S)-isodihydrocarvone + NAD+ plus about 20% of (1S,4S)-dihydrocarvone ?
1.3.1.92 2-cyclohexen-1-one + NADH + H+
-
 Artemisia annua cyclohexanone + NAD+
-
 ?
1.3.1.92 2E-nonenal + NADH + H+ about 12% of the activity with artemisinic aldehyde Artemisia annua nonanal + NAD+
-
 ?
1.3.1.92 artemisinic aldehyde + NADH + H+
-
 Artemisia annua (11R)-dihydroartemisinic aldehyde + NAD+
-
 ?
1.3.1.92 artemisinic aldehyde + NADPH + H+
-
 Artemisia annua dihydroartemisinic aldehyde + NADP+
-
 ?
1.3.1.92 additional information no substrate: arteannuin B, artemisinic acid, artemisinic alcohol, artemisitene, coniferyl aldehyde, (2E)-nonenal, alpha-pinene, (+)-pulegone, and sabinone Artemisia annua ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.92 Dbr2
-
 Artemisia annua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.92 0.86
-
 (+)-carvone 30°C, pH 7.5 Artemisia annua
1.3.1.92 1.3
-
 NADH 30°C, pH 7.5 Artemisia annua
1.3.1.92 1.8
-
 2-cyclohexen-1-one 30°C, pH 7.5 Artemisia annua
1.3.1.92 2.5
-
 NADPH 30°C, pH 7.5 Artemisia annua
1.3.1.92 2.6
-
 artemisinic aldehyde 30°C, pH 7.5 Artemisia annua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.92 7.5
-
-
 Artemisia annua

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.92 5.5
-
 complete inhibition Artemisia annua
1.3.1.92 9
-
 about 25% of maximum activity Artemisia annua

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.92 NADH NADPH is preferred over NADH Artemisia annua
1.3.1.92 NADPH NADPH is preferred over NADH Artemisia annua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.92 1.3
-
 (+)-carvone 30°C, pH 7.5 Artemisia annua
1.3.1.92 1.7
-
 NADH 30°C, pH 7.5 Artemisia annua
1.3.1.92 2.3
-
 2-cyclohexen-1-one 30°C, pH 7.5 Artemisia annua
1.3.1.92 26
-
 NADPH 30°C, pH 7.5 Artemisia annua
1.3.1.92 140
-
 artemisinic aldehyde 30°C, pH 7.5 Artemisia annua