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Literature summary extracted from

  • Khoo, S.K.; Loll, B.; Chan, W.T.; Shoeman, R.L.; Ngoo, L.; Yeo, C.C.; Meinhart, A.
    Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae (2007), J. Biol. Chem., 282, 19606-19618.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.176 gene pezT, DNA and amino acid sequence determination and analysis, sequence comparison, co-expression of fused His-tagged PezT with His-tagged PezA in Escherichia coli BL21(DE3). The PezA antitoxin represses transcription from PpezAT and the PezT toxin acts as a co-repressor Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.176 D66T site-directed mutagenesis Streptococcus pneumoniae
2.7.1.176 K45A site-directed mutagenesis, the mutation abolishes PezT lethality Streptococcus pneumoniae
2.7.1.176 R157A site-directed mutagenesis, the mutation abolishes PezT lethality Streptococcus pneumoniae
2.7.1.176 R170A site-directed mutagenesis, the mutation abolishes PezT lethality Streptococcus pneumoniae
2.7.1.176 T117V site-directed mutagenesis, the mutation abolishes PezT lethality Streptococcus pneumoniae
2.7.1.176 T120V site-directed mutagenesis, the mutation abolishes PezT lethality and a delay in the growth inhibition Streptococcus pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.176 Streptococcus pneumoniae Q97QZ1 gene pezT
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.176 recombinant fused His-tagged PezT with His-tagged PezA from Escherichia coli BL21(DE3) by nickel affinity chromatography, PezA is cleaved off by thrombin, followed by anion exchange and gel filtration to purifiy PezT and PezA, quantitative real-time RT PCR expression analysis Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.176 additional information phosphoryltransfer reaction from ATP/GTP to an unknown substrate is much more likely responsible for toxicity than a simple nucleoside triphosphate hydrolysis Streptococcus pneumoniae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.176 PezT
-
 Streptococcus pneumoniae

General Information

EC Number General Information Comment Organism
2.7.1.176 metabolism expression of PezT and zeta in Escherichia coli lead to temporary inhibition in cell growth and is therefore seen as triggering cell stasis rather than cell death Streptococcus pneumoniae
2.7.1.176 physiological function PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae, the toxicity of PezT is counteracted by PezA, which is encoded immediately upstream of pezT and shares weak sequence similarities in the C-terminal region with the epsilon antitoxin. The pezAT genes form a bicistronic operon that is co-transcribed from a sigma70-like promoter upstream of pezA and is negatively autoregulated with PezA functioning as a transcriptional repressor and PezT as a co-repressor. Inactivation of PezT by PezA, in the inactive PezA2PezT2 heterotetrameric protein complex, the nucleotide binding site of PezT is covered by two helices of PezA Streptococcus pneumoniae