Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Soares, J.; Zhang, L.; Pitsch, R.; Kleinholz, N.; Jones, R.; Wolff, J.; Amster, J.; Green-Church, K.; Krzyck, J.
    The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases (2005), J. Biol. Chem., 280, 36962-36969.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.248 gene mtmB Methanosarcina barkeri
2.1.1.249 gene mtbB Methanosarcina barkeri
2.1.1.250 gene mttB, single gene Methanosarcina barkeri

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.248 crystal structure determination and analysis Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.248 50000
-
 x * 50000, SDS-PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri MS / DSM 800
-
 a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
2.1.1.249 dimethylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 dimethylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri MS / DSM 800
-
 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanosarcina barkeri MS / DSM 800
-
 a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.248 Methanosarcina barkeri O30642 gene mtmB1; gene mtmB1
-
2.1.1.248 Methanosarcina barkeri Q9P9L4 gene mtmB2; gene mtmB2
-
2.1.1.248 Methanosarcina barkeri MS / DSM 800 O30642 gene mtmB1; gene mtmB1
-
2.1.1.248 Methanosarcina barkeri MS / DSM 800 Q9P9L4 gene mtmB2; gene mtmB2
-
2.1.1.249 Methanosarcina barkeri Q9P9N0 gene mtbB
-
2.1.1.249 Methanosarcina barkeri MS / DSM 800 Q9P9N0 gene mtbB
-
2.1.1.250 Methanosarcina barkeri O93658 gene mttB
-
2.1.1.250 Methanosarcina barkeri MS / DSM 800 O93658 gene mttB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.248 native enzyme, separation from co-purifiying MtmC, the methylamine-specific corrinoid protein, by gel filtration Methanosarcina barkeri
2.1.1.249 native enzyme by three different steps of anion exchange chromatography, adsorption and hydrophobic interaction chromatography Methanosarcina barkeri
2.1.1.250 native enzyme as the MttB-MttC complex by repeated gel filtration Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri MS / DSM 800 a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri MS / DSM 800 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 dimethylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 dimethylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri MS / DSM 800 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri MS / DSM 800 a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.248 ? x * 50000, SDS-PAGE Methanosarcina barkeri
2.1.1.248 More mass spectrometric structure analysis, overview, primary structure of MtmB Methanosarcina barkeri
2.1.1.249 More mass spectrometric structure analysis, overview, primary structure of MtbB Methanosarcina barkeri
2.1.1.250 More mass spectrometric structure analysis, overview, primary structure of MttB Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.248 MtmB
-
 Methanosarcina barkeri
2.1.1.248 mtmB1
-
 Methanosarcina barkeri
2.1.1.248 mtmB2
-
 Methanosarcina barkeri
2.1.1.249 dimethylamine:CoM methyltransferase
-
 Methanosarcina barkeri
2.1.1.249 DMA methyltransferase
-
 Methanosarcina barkeri
2.1.1.249 MtbB
-
 Methanosarcina barkeri
2.1.1.250 MttB
-
 Methanosarcina barkeri
2.1.1.250 TMA methyltransferase
-
 Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.248 additional information single in-frame amber UAG codons are found in the genes encoding MtmB, MtbB, or MttB, the methyltransferases initiating methane formation from monomethylamine, dimethylamine, or trimethylamine, respectively, in certain Archaea. The amber codon codes for pyrrolysine, the 22nd genetically encoded amino acid found in nature Methanosarcina barkeri
2.1.1.249 additional information single in-frame amber UAG codons are found in the genes encoding MtmB, MtbB, or MttB, the methyltransferases initiating methane formation from monomethylamine, dimethylamine, or trimethylamine, respectively, in certain Archaea. The amber codon codes for pyrrolysine, the 22nd genetically encoded amino acid found in nature Methanosarcina barkeri
2.1.1.250 additional information single in-frame amber UAG codons are found in the genes encoding MtmB, MtbB, or MttB, the methyltransferases initiating methane formation from monomethylamine, dimethylamine, or trimethylamine, respectively, in certain Archaea. The amber codon codes for pyrrolysine, the 22nd genetically encoded amino acid found in nature Methanosarcina barkeri