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Literature summary extracted from

  • Burke, S.; Krzycki, J.
    Reconstitution of monomethylamine:coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri (1997), J. Biol. Chem., 272, 16570-16577.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.248 52000
-
 3 * 52000 Methanosarcina barkeri
2.1.1.248 170000
-
-
 Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.248 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.248 native MtmB from monomethylamine-grown cells, by two different steps of anionexchange chromatography, followed by gel filtration and another step of anion exchange chromatography to near homogeneity Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.248 0.49
-
 purified native enzyme, pH 7.0, 37°C Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.248 homotrimer 3 * 52000 Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.248 MMA:MMCP methyltransferase
-
 Methanosarcina barkeri
2.1.1.248 MMAMT
-
 Methanosarcina barkeri
2.1.1.248 monomethylamine:coenzyme M methyl transferase
-
 Methanosarcina barkeri
2.1.1.248 monomethylamine:MMCP methyltransferase
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.248 37
-
 assay at Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.248 7
-
 assay at Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.248 physiological function the MMAMT polypeptide is rate-limiting for methyl transfer until at a 2fold molar excess over monomethylamine corrinoid protein, MMCP. MMAMT and MMCP form a complex. MMCP is the major corrinoid protein for methanogenesis from monomethylamine Methanosarcina barkeri