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Literature summary extracted from
- Purification and characterization of vitamin D 25-hydroxylase from rat liver mitochondria (1988), J. Biol. Chem., 263, 14256-14260.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.24 | Metyrapone | slight inhibition | Rattus norvegicus |  |
| 1.14.14.24 | additional information | not inhibitory: aminoglutethimide. The enzyme activity was markedly inhibited in an atmosphere of CO:Os:Nz, 40:20:40 | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.24 | 0.054 | - |
1alpha-hydroxycholecalciferol | pH 7.4, 37°C | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.24 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.24 | 52500 | - |
x * 52500, SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.24 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.24 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.24 | liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.24 | 1alpha-hydroxycholecalciferol + O2 + NADPH + H+ | - |
Rattus norvegicus | 1alpha,25-dihydroxycholecalciferol + NADP+ + H2O | - |
? | |
| 1.14.14.24 | cholecalciferol + O2 + NADPH + H+ | at about 20% of the rate with 1alpha-hydroxycholecalciferol | Rattus norvegicus | 25-hydroxycholecalciferol + NADP+ + H2O | - |
? | |
| 1.14.14.24 | additional information | no substrates: benzphetamine, 7-ethoxycoumarin, and benzo[a]pyrene | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.24 | ? | x * 52500, SDS-PAGE | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.24 | 0.063 | - |
1alpha-hydroxycholecalciferol | pH 7.4, 37°C | Rattus norvegicus | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.24 | cytochrome P450 | - |
Rattus norvegicus | |
| 1.14.14.24 | additional information | activity is completely lost when the electron-transferring system is replaced by that of microsomes, i.e. NADPH-cytochrome P-450 reductase purified from rat liver microsomes. The omission of cytochrome P-450, adrenodoxin, or NADPH-adrenodoxin reductase results in complete loss of enzyme activity | Rattus norvegicus | |
| 1.14.14.24 | NADPH | - |
Rattus norvegicus | |
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