EC Number | General Stability | Organism |
---|---|---|
1.1.1.324 | the enzyme rapidly and irreversibly loses its activity when 1 mM dithiothreitol and 20% glycerol v/v are omitted from the enzyme solution | Rauvolfia serpentina |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.324 | iridodial | - |
Rauvolfia serpentina | |
1.1.1.324 | isopulegol | - |
Rauvolfia serpentina | |
1.1.1.324 | menthol | competitive against 8-hydroxygeraniol | Rauvolfia serpentina |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.324 | additional information | - |
additional information | steady-state kinetic studies show that the nerol dehydrogenation proceeds by an ordered Bi-Bi mechanism, NADP+ binds the enzyme first and then NADPH is the second product released from it, kinetic mechanism, overview | Rauvolfia serpentina |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.324 | additional information | the enzyme contains no Mo, Co, Cu, Fe, Mn, Mg, Ca, Se, Pt, and Au | Rauvolfia serpentina | |
1.1.1.324 | Zn2+ | the enzyme contains zinc ions | Rauvolfia serpentina |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.324 | 44000 | - |
1 * 44000, SDS-PAGE | Rauvolfia serpentina |
1.1.1.324 | 50000 | - |
native enzyme, gel filtration | Rauvolfia serpentina |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.324 | (6E)-8-hydroxygeraniol + 2 NADP+ | Rauvolfia serpentina | 10-hydroxygeraniol undergoes a reversible dehydrogenation to produce 8-oxogeraniol or 8-hydroxygeranial, which are oxidized further to give 8-oxogeranial, the direct precursor of iridodial | (6E)-8-oxogeranial + 2 NADPH + 2 H+ | - |
r | |
1.1.1.324 | additional information | Rauvolfia serpentina | various branched-chain allylic primary alcohols, such as nerol, geraniol, and 8-hydroxygeraniol, are substrates, but ethanol is inert. The enzyme is also active with farnesol, 3-methyl-2-buten1-ol, citronellol, 4-isopropylbenzyl alcohol, trans-2-heptenol, and trans-2-hexenol. The enzyme exclusively transfers the pro-R hydrogen of NADPH to neral. No activity with linalool, 2,6-dimethyl-octane, iridodial, 1-octanol, 1-heptanol, 1-pentanol, 3-methyl-3-buten-1-ol, trans-crotyl alcohol, isopulegol, and menthol | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.324 | Rauvolfia serpentina | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.324 | native enzyme to homogeneity by ammonium sulfate fractionation, hydrophobic interaction, anion exchange, and AF-Red dye affinity chromatography followed by gel filtration | Rauvolfia serpentina |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.324 | 2.7 | - |
purified enzyme, pH 9.0, 25°C | Rauvolfia serpentina |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.324 | -20°C, purified enzyme, 20 mM Tris HCl, pH 8.0, 20% glycerol, 1 mM DTT, 6 months without loss of activity | Rauvolfia serpentina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.324 | (6E)-8-hydroxygeraniol + 2 NADP+ | - |
Rauvolfia serpentina | (6E)-8-oxogeranial + 2 NADPH + 2 H+ | - |
r | |
1.1.1.324 | (6E)-8-hydroxygeraniol + 2 NADP+ | 10-hydroxygeraniol undergoes a reversible dehydrogenation to produce 8-oxogeraniol or 8-hydroxygeranial, which are oxidized further to give 8-oxogeranial, the direct precursor of iridodial | Rauvolfia serpentina | (6E)-8-oxogeranial + 2 NADPH + 2 H+ | - |
r | |
1.1.1.324 | (6E)-8-hydroxynerol + 2 NADP+ | - |
Rauvolfia serpentina | ? + 2 NADPH + 2 H+ | - |
r | |
1.1.1.324 | geraniol + 2 NADP+ | - |
Rauvolfia serpentina | ? + 2 NADPH + 2 H+ | - |
r | |
1.1.1.324 | additional information | various branched-chain allylic primary alcohols, such as nerol, geraniol, and 8-hydroxygeraniol, are substrates, but ethanol is inert. The enzyme is also active with farnesol, 3-methyl-2-buten1-ol, citronellol, 4-isopropylbenzyl alcohol, trans-2-heptenol, and trans-2-hexenol. The enzyme exclusively transfers the pro-R hydrogen of NADPH to neral. No activity with linalool, 2,6-dimethyl-octane, iridodial, 1-octanol, 1-heptanol, 1-pentanol, 3-methyl-3-buten-1-ol, trans-crotyl alcohol, isopulegol, and menthol | Rauvolfia serpentina | ? | - |
? | |
1.1.1.324 | nerol + 2 NADP+ | - |
Rauvolfia serpentina | ? + 2 NADPH + 2 H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.324 | monomer | 1 * 44000, SDS-PAGE | Rauvolfia serpentina |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.324 | acyclic monoterpene primary alcohol:NADP+ oxidoreductase | - |
Rauvolfia serpentina |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.324 | 25 | - |
assay at | Rauvolfia serpentina |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.324 | 7 | - |
reverse reaction assay at | Rauvolfia serpentina |
1.1.1.324 | 9 | - |
forward reaction assay at | Rauvolfia serpentina |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.324 | NADP+ | the enzyme exclusively requires NADP+ or NADPH as the cofactor | Rauvolfia serpentina | |
1.1.1.324 | NADPH | the enzyme exclusively requires NADP+ or NADPH as the cofactor | Rauvolfia serpentina |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.324 | 0.53 | - |
menthol | pH 9.0, 25°C, versus 8-hydroxygeraniol | Rauvolfia serpentina |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.324 | Rauvolfia serpentina | isoelectric focusing | - |
5.4 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.324 | metabolism | the acyclic monoterpene primary alcohol:NADP+ oxidoreductase of Rauwolfia serpentina is a key enzyme in biosynthesis of monoterpene alcohols in the plant | Rauvolfia serpentina |