EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.249 | gene mtbB2, DNA and amino acid sequence determination and analysis, homology sequence comparison, co-transcription with of gene mttB encoding the TMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases | Methanosarcina barkeri |
2.1.1.249 | gene mtbB3, DNA and amino acid sequence determination and analysis, homology sequence comparison, co-transcription with of gene mttB encoding the TMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases | Methanosarcina barkeri |
2.1.1.249 | genes mtbB1, mtbB2, and mtbB3, DNA and amino acid sequence determination and analysis, homology sequence comparison, co-transcription with of gene mttB encoding the TMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases | Methanosarcina barkeri |
2.1.1.250 | gene mttB, co-transcription with gene mtbB1 encoding the DMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes of all methyltransferases each contain a single in-frame amber codon | Methanosarcina barkeri |
2.1.1.250 | gene mttB, co-transcription with gene mtbB1 encoding the DMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes of all methyltransferases each contain a single in-frame amber codon | Methanosarcina thermophila |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.250 | additional information | the determined N-terminus of MttB, encoded by mttB, shows a UAG codon, which is represented in both strands of the DNA, follows at codon position 334. Cessation of translation at the UAG codon results in a 34-kDa product rather than the abundant 53-kDa polypeptide purified from TMA-grown cells | Methanosarcina barkeri |
2.1.1.250 | additional information | the determined N-terminus of MttB, encoded by mttB, shows a UAG codon, which is represented in both strands of the DNA, follows at codon position 334. Cessation of translation at the UAG codon results in a 34-kDa product rather than the abundant 53-kDa polypeptide purified from TMA-grown cells | Methanosarcina thermophila |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.249 | 50000 | - |
x * 50000, SDS-PAGE | Methanosarcina barkeri |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.249 | dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
2.1.1.250 | trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
2.1.1.250 | trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | Methanosarcina thermophila | - |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.249 | Methanosarcina barkeri | O93661 | MtbB1; genes mtbB1, mtbB2, or mtbB3 | - |
2.1.1.249 | Methanosarcina barkeri | Q9P9M9 | MtbB3; gene mtbB3 | - |
2.1.1.249 | Methanosarcina barkeri | Q9P9N0 | mtbB2; gene mtbB2 | - |
2.1.1.250 | Methanosarcina barkeri | O93658 | MttB; single copy gene mttB | - |
2.1.1.250 | Methanosarcina thermophila | Q9P995 | MttB; gene mttB | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.249 | dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
2.1.1.249 | dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | the DMA methyltransferase cognate corrinoid protein MtbC | Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
2.1.1.250 | trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
2.1.1.250 | trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | - |
Methanosarcina thermophila | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
2.1.1.250 | trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | the TMA methyltransferase cognate corrinoid protein MttC | Methanosarcina barkeri | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
2.1.1.250 | trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | the TMA methyltransferase cognate corrinoid protein MttC | Methanosarcina thermophila | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.249 | ? | x * 50000, SDS-PAGE | Methanosarcina barkeri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.249 | dimethylamine methyltransferase | - |
Methanosarcina barkeri |
2.1.1.249 | DMA methyltransferase | - |
Methanosarcina barkeri |
2.1.1.249 | MtbB | - |
Methanosarcina barkeri |
2.1.1.249 | MtbB2 | - |
Methanosarcina barkeri |
2.1.1.249 | MtbB3 | - |
Methanosarcina barkeri |
2.1.1.250 | MttB | - |
Methanosarcina barkeri |
2.1.1.250 | MttB | - |
Methanosarcina thermophila |
2.1.1.250 | TMA methyltransferase | - |
Methanosarcina barkeri |
2.1.1.250 | TMA methyltransferase | - |
Methanosarcina thermophila |
2.1.1.250 | trimethylamine methyltransferase | - |
Methanosarcina barkeri |
2.1.1.250 | trimethylamine methyltransferase | - |
Methanosarcina thermophila |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.249 | metabolism | three different methyltransferases initiate methanogenesis from trimethylamine, dimethylamine, or monomethylamine by methylating different cognate corrinoid proteins that are subsequently used to methylate coenzyme M | Methanosarcina barkeri |
2.1.1.250 | metabolism | three different methyltransferases initiate methanogenesis from trimethylamine, dimethylamine, or monomethylamine by methylating different cognate corrinoid proteins that are subsequently used to methylate coenzyme M | Methanosarcina barkeri |
2.1.1.250 | metabolism | three different methyltransferases initiate methanogenesis from trimethylamine, dimethylamine, or monomethylamine by methylating different cognate corrinoid proteins that are subsequently used to methylate coenzyme M | Methanosarcina thermophila |