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Literature summary extracted from

  • Paul, L.; Ferguson Jr., D.; Krzycki, J.
    The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons (2000), J. Bacteriol., 182, 2520-2529.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.249 gene mtbB2, DNA and amino acid sequence determination and analysis, homology sequence comparison, co-transcription with of gene mttB encoding the TMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases Methanosarcina barkeri
2.1.1.249 gene mtbB3, DNA and amino acid sequence determination and analysis, homology sequence comparison, co-transcription with of gene mttB encoding the TMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases Methanosarcina barkeri
2.1.1.249 genes mtbB1, mtbB2, and mtbB3, DNA and amino acid sequence determination and analysis, homology sequence comparison, co-transcription with of gene mttB encoding the TMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases Methanosarcina barkeri
2.1.1.250 gene mttB, co-transcription with gene mtbB1 encoding the DMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes of all methyltransferases each contain a single in-frame amber codon Methanosarcina barkeri
2.1.1.250 gene mttB, co-transcription with gene mtbB1 encoding the DMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes of all methyltransferases each contain a single in-frame amber codon Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.250 additional information the determined N-terminus of MttB, encoded by mttB, shows a UAG codon, which is represented in both strands of the DNA, follows at codon position 334. Cessation of translation at the UAG codon results in a 34-kDa product rather than the abundant 53-kDa polypeptide purified from TMA-grown cells Methanosarcina barkeri
2.1.1.250 additional information the determined N-terminus of MttB, encoded by mttB, shows a UAG codon, which is represented in both strands of the DNA, follows at codon position 334. Cessation of translation at the UAG codon results in a 34-kDa product rather than the abundant 53-kDa polypeptide purified from TMA-grown cells Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.249 50000
-
x * 50000, SDS-PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] Methanosarcina barkeri
-
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanosarcina barkeri
-
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanosarcina thermophila
-
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.249 Methanosarcina barkeri O93661 MtbB1; genes mtbB1, mtbB2, or mtbB3
-
2.1.1.249 Methanosarcina barkeri Q9P9M9 MtbB3; gene mtbB3
-
2.1.1.249 Methanosarcina barkeri Q9P9N0 mtbB2; gene mtbB2
-
2.1.1.250 Methanosarcina barkeri O93658 MttB; single copy gene mttB
-
2.1.1.250 Methanosarcina thermophila Q9P995 MttB; gene mttB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] the DMA methyltransferase cognate corrinoid protein MtbC Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
Methanosarcina barkeri a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
Methanosarcina thermophila a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] the TMA methyltransferase cognate corrinoid protein MttC Methanosarcina barkeri a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] the TMA methyltransferase cognate corrinoid protein MttC Methanosarcina thermophila a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.249 ? x * 50000, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.249 dimethylamine methyltransferase
-
Methanosarcina barkeri
2.1.1.249 DMA methyltransferase
-
Methanosarcina barkeri
2.1.1.249 MtbB
-
Methanosarcina barkeri
2.1.1.249 MtbB2
-
Methanosarcina barkeri
2.1.1.249 MtbB3
-
Methanosarcina barkeri
2.1.1.250 MttB
-
Methanosarcina barkeri
2.1.1.250 MttB
-
Methanosarcina thermophila
2.1.1.250 TMA methyltransferase
-
Methanosarcina barkeri
2.1.1.250 TMA methyltransferase
-
Methanosarcina thermophila
2.1.1.250 trimethylamine methyltransferase
-
Methanosarcina barkeri
2.1.1.250 trimethylamine methyltransferase
-
Methanosarcina thermophila

General Information

EC Number General Information Comment Organism
2.1.1.249 metabolism three different methyltransferases initiate methanogenesis from trimethylamine, dimethylamine, or monomethylamine by methylating different cognate corrinoid proteins that are subsequently used to methylate coenzyme M Methanosarcina barkeri
2.1.1.250 metabolism three different methyltransferases initiate methanogenesis from trimethylamine, dimethylamine, or monomethylamine by methylating different cognate corrinoid proteins that are subsequently used to methylate coenzyme M Methanosarcina barkeri
2.1.1.250 metabolism three different methyltransferases initiate methanogenesis from trimethylamine, dimethylamine, or monomethylamine by methylating different cognate corrinoid proteins that are subsequently used to methylate coenzyme M Methanosarcina thermophila