EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.33 | 0.0085 | - |
ethylenediaminetetraacetate | presence of Mg2+, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 | |
1.14.14.33 | 0.034 | - |
ethylenediaminetetraacetate | no addition of divalent ions, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.10 | Mg2+ | EDTA monooxygenase degrades nitrilotriacetate in the absence or presence of Mg2+ | Chelativorans sp. BNC1 | |
1.14.14.33 | Mg2+ | MgEDTA2- is the best substrate | EDTA-degrading bacterium BNC1 | |
1.14.14.33 | additional information | the enzyme is able to release glyoxylate from EDTA complexed with any of the cations tested (Mg2+, Mn2+, Ni2+, Co2+, Zn2+, Fe2+, Ca2+, Cu2+, Cr2+, Sn2+, Ba2+, Cd2+, Sr2+, Pd2+, Al3+, Cr3+, K+, or Na+). MgEDTA2- is the best substrate | EDTA-degrading bacterium BNC1 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.10 | 45000 | - |
gel filtration | Chelativorans sp. BNC1 |
1.14.14.33 | 45000 | - |
gel filtration | EDTA-degrading bacterium BNC1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.10 | Chelativorans sp. BNC1 | - |
- |
- |
1.14.14.33 | EDTA-degrading bacterium BNC1 | Q9F9T3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.10 | ammonium sulfate precipitation, Blue 3GA agarose column chromatography, MonoQ HR 5/5 column chromatography, and hydroxyapatite column chromatography | Chelativorans sp. BNC1 |
1.14.14.33 | - |
EDTA-degrading bacterium BNC1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.10 | EDTA + O2 + FMNH2 + H+ | - |
Chelativorans sp. BNC1 | ethylenediaminetriacetate + glyoxylate + H2O + FMN | - |
? | |
1.14.14.10 | nitrilotriacetate + O2 + FMNH2 + H+ | - |
Chelativorans sp. BNC1 | iminodiacetate + glyoxylate + H2O + FMN | - |
? | |
1.14.14.33 | diethylenetriaminepentaacetate + FMNH2 + O2 | - |
EDTA-degrading bacterium BNC1 | diethylenetriaminetetraacetate + glyoxylate + FMN + H2O | - |
? | |
1.14.14.33 | ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | - |
EDTA-degrading bacterium BNC1 | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | - |
? | |
1.14.14.33 | additional information | the enzyme oxidizes both EDTA complexed with various metal ions and uncomplexed EDTA | EDTA-degrading bacterium BNC1 | ? | - |
? | |
1.14.14.33 | nitrilotriacetate + FMNH2 + O2 | - |
EDTA-degrading bacterium BNC1 | nitrilodiacetate + glyoxylate + FMN + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.10 | monomer | 1 * 45000, SDS-PAGE | Chelativorans sp. BNC1 |
1.14.14.33 | monomer | 1 * 45000, SDS-PAGE | EDTA-degrading bacterium BNC1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.10 | EDTA monooxygenase | - |
Chelativorans sp. BNC1 |
1.14.14.10 | NTA-MO | - |
Chelativorans sp. BNC1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.33 | 35 | - |
- |
EDTA-degrading bacterium BNC1 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.33 | 25 | - |
75% of maximum activity | EDTA-degrading bacterium BNC1 |
1.14.14.33 | 40 | - |
96% of maximum activity | EDTA-degrading bacterium BNC1 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.33 | 2.8 | - |
ethylenediaminetetraacetate | no addition of divalent ions, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 | |
1.14.14.33 | 3.6 | - |
ethylenediaminetetraacetate | presence of Mg2+, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.33 | 7.7 | - |
- |
EDTA-degrading bacterium BNC1 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.33 | 6.9 | - |
75% of maximum activity | EDTA-degrading bacterium BNC1 |
1.14.14.33 | 8.1 | - |
70% of maximum activity | EDTA-degrading bacterium BNC1 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.10 | FMNH2 | - |
Chelativorans sp. BNC1 | |
1.14.14.33 | FMNH2 | - |
EDTA-degrading bacterium BNC1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.33 | 80 | - |
ethylenediaminetetraacetate | no addition of divalent ions, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 | |
1.14.14.33 | 430 | - |
ethylenediaminetetraacetate | presence of Mg2+, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 |