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Literature summary extracted from

  • Payne, J.W.; Bolton, H.; Campbell, J.A.; Xun, L.
    Purification and characterization of EDTA monooxygenase from the EDTA-degrading bacterium BNC1 (1998), J. Bacteriol., 180, 3823-3827.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.33 0.0085
-
ethylenediaminetetraacetate presence of Mg2+, pH 7.8, 35°C EDTA-degrading bacterium BNC1
1.14.14.33 0.034
-
ethylenediaminetetraacetate no addition of divalent ions, pH 7.8, 35°C EDTA-degrading bacterium BNC1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.10 Mg2+ EDTA monooxygenase degrades nitrilotriacetate in the absence or presence of Mg2+ Chelativorans sp. BNC1
1.14.14.33 Mg2+ MgEDTA2- is the best substrate EDTA-degrading bacterium BNC1
1.14.14.33 additional information the enzyme is able to release glyoxylate from EDTA complexed with any of the cations tested (Mg2+, Mn2+, Ni2+, Co2+, Zn2+, Fe2+, Ca2+, Cu2+, Cr2+, Sn2+, Ba2+, Cd2+, Sr2+, Pd2+, Al3+, Cr3+, K+, or Na+). MgEDTA2- is the best substrate EDTA-degrading bacterium BNC1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.10 45000
-
gel filtration Chelativorans sp. BNC1
1.14.14.33 45000
-
gel filtration EDTA-degrading bacterium BNC1

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.10 Chelativorans sp. BNC1
-
-
-
1.14.14.33 EDTA-degrading bacterium BNC1 Q9F9T3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.10 ammonium sulfate precipitation, Blue 3GA agarose column chromatography, MonoQ HR 5/5 column chromatography, and hydroxyapatite column chromatography Chelativorans sp. BNC1
1.14.14.33
-
EDTA-degrading bacterium BNC1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.10 EDTA + O2 + FMNH2 + H+
-
Chelativorans sp. BNC1 ethylenediaminetriacetate + glyoxylate + H2O + FMN
-
?
1.14.14.10 nitrilotriacetate + O2 + FMNH2 + H+
-
Chelativorans sp. BNC1 iminodiacetate + glyoxylate + H2O + FMN
-
?
1.14.14.33 diethylenetriaminepentaacetate + FMNH2 + O2
-
EDTA-degrading bacterium BNC1 diethylenetriaminetetraacetate + glyoxylate + FMN + H2O
-
?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2
-
EDTA-degrading bacterium BNC1 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O
-
?
1.14.14.33 additional information the enzyme oxidizes both EDTA complexed with various metal ions and uncomplexed EDTA EDTA-degrading bacterium BNC1 ?
-
?
1.14.14.33 nitrilotriacetate + FMNH2 + O2
-
EDTA-degrading bacterium BNC1 nitrilodiacetate + glyoxylate + FMN + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.10 monomer 1 * 45000, SDS-PAGE Chelativorans sp. BNC1
1.14.14.33 monomer 1 * 45000, SDS-PAGE EDTA-degrading bacterium BNC1

Synonyms

EC Number Synonyms Comment Organism
1.14.14.10 EDTA monooxygenase
-
Chelativorans sp. BNC1
1.14.14.10 NTA-MO
-
Chelativorans sp. BNC1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.33 35
-
-
EDTA-degrading bacterium BNC1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.14.33 25
-
75% of maximum activity EDTA-degrading bacterium BNC1
1.14.14.33 40
-
96% of maximum activity EDTA-degrading bacterium BNC1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.33 2.8
-
ethylenediaminetetraacetate no addition of divalent ions, pH 7.8, 35°C EDTA-degrading bacterium BNC1
1.14.14.33 3.6
-
ethylenediaminetetraacetate presence of Mg2+, pH 7.8, 35°C EDTA-degrading bacterium BNC1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.33 7.7
-
-
EDTA-degrading bacterium BNC1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.14.33 6.9
-
75% of maximum activity EDTA-degrading bacterium BNC1
1.14.14.33 8.1
-
70% of maximum activity EDTA-degrading bacterium BNC1

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.10 FMNH2
-
Chelativorans sp. BNC1
1.14.14.33 FMNH2
-
EDTA-degrading bacterium BNC1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.33 80
-
ethylenediaminetetraacetate no addition of divalent ions, pH 7.8, 35°C EDTA-degrading bacterium BNC1
1.14.14.33 430
-
ethylenediaminetetraacetate presence of Mg2+, pH 7.8, 35°C EDTA-degrading bacterium BNC1