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Literature summary extracted from

  • Ferguson Jr., D.; Krzycki, J.
    Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri (1997), J. Bacteriol., 179, 846-852.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.247 methyl viologen strictly dependent on Methanosarcina barkeri
2.1.1.247 additional information stimulation of the TMA:CoM methyl transfer reaction in cell extracts Methanosarcina barkeri
2.1.1.247 Ti(III) citrate strictly dependent on Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.247 52000
-
 1 * 52000, SDS-PAGE Methanosarcina barkeri
2.1.1.247 52900
-
 gel filtration and native PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.247 additional information Methanosarcina barkeri corrinoid proteins mediating CoM methylation from dimethylamine or monomethylamine have a specific requirement for MT2-A, reconstitution of trimethylamine-dependent coenzyme M methylation, overview. Trimethylamine methyl transfer can interact with either isozyme of MT2 but has the greatest affinity for the A isozyme. The predominant isozyme of MT2 from in TMA-grown cells is the favored participant in the TMA:CoM methyl transfer reaction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.247 Methanosarcina barkeri O30640 amine isozyme MT2-A, gene mtbA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.247 native enzyme by two different steps of anion exchange chromatography, gel filtration, and another step of anion exchange chromatography, co-purification with the 26-kDa the trimethylamine-specific corrinoid protein Methanosarcina barkeri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.247 culture condition:trimethylamine-grown cell
-
 Methanosarcina barkeri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.247 1.7
-
 isozyme MT2-A, pH 7.0, 37°C Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.247 a [methyl-Co(III) methylamine-specific corrinoid protein] + coenzyme M trimethylamine methyl transfer required, neither dimethylamine nor monomethylamine serve as substrate Methanosarcina barkeri methyl-CoM + a [Co(I) methylamine-specific corrinoid protein]
-
 ?
2.1.1.247 additional information corrinoid proteins mediating CoM methylation from dimethylamine or monomethylamine have a specific requirement for MT2-A, reconstitution of trimethylamine-dependent coenzyme M methylation, overview. Trimethylamine methyl transfer can interact with either isozyme of MT2 but has the greatest affinity for the A isozyme. The predominant isozyme of MT2 from in TMA-grown cells is the favored participant in the TMA:CoM methyl transfer reaction Methanosarcina barkeri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.247 monomer 1 * 52000, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.247 methylcorrinoid:CoM methyltransferase
-
 Methanosarcina barkeri
2.1.1.247 methyltransferase II
-
 Methanosarcina barkeri
2.1.1.247 MT2
-
 Methanosarcina barkeri
2.1.1.247 TMA:CoM methyltransferase
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.247 37
-
 assay at Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.247 7
-
 assay at Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.247 metabolism involvement of MT2-A in monomethylamine metabolism Methanosarcina barkeri