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Literature summary extracted from
- Camphor revisited: Involvement of a unique monooxygenase in metabolism of 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid by Pseudomonas putida (1983), J. Bacteriol., 153, 140-152.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.13.160 | additional information | the ability to grow on (+)-camphor is transferred to a recipient strain, Pseudomonas putida PaW340, which is a streptomycin-resistant tryptophan auxotroph that is derived from Pseudomonas putida (arvilla) mt-2 | Pseudomonas putida |
| 6.2.1.38 | additional information | the ability to grow on (+)-camphor is transferred to a recipient strain, Pseudomonas putida PaW340, which is a streptomycin-resistant tryptophan auxotroph that is derived from Pseudomonas putida (arvilla) mt-2 | Pseudomonas putida |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.160 | additional information | the enzyme contains no iron | Pseudomonas putida | |
| 1.14.13.160 | additional information | protein contains less than 0.1 atom of Fe per molecule | Pseudomonas putida | |
| 6.2.1.38 | Mg2+ | required | Pseudomonas putida |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.160 | 56000 | - |
2 * 56000, SDS-PAGE | Pseudomonas putida |
| 1.14.13.160 | 106000 | - |
gel filtration | Pseudomonas putida |
| 1.14.13.160 | 106000 | - |
analytical sedimentation centrifugation | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ | Pseudomonas putida | - |
[(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O | - |
? | |
| 1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ | Pseudomonas putida C1 / ATCC 17453 | - |
[(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O | - |
? | |
| 6.2.1.38 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA | Pseudomonas putida | - |
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA | - |
? | |
| 6.2.1.38 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA | Pseudomonas putida C1 / ATCC 17453 | - |
AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.160 | no activity in Pseudomonas putida strain PaW340 | - |
- |
- |
| 1.14.13.160 | Pseudomonas putida | - |
- |
- |
| 1.14.13.160 | Pseudomonas putida C1 / ATCC 17453 | - |
- |
- |
| 6.2.1.38 | no activity in Pseudomonas putida strain PaW340 | - |
- |
- |
| 6.2.1.38 | Pseudomonas putida | - |
- |
- |
| 6.2.1.38 | Pseudomonas putida C1 / ATCC 17453 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.160 | - |
Pseudomonas putida |
| 1.14.13.160 | native enzyme 84fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, again ammonium sulfate fractionation, and affinity chromatography | Pseudomonas putida |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.160 | culture condition:(+)-camphor-grown cell | inducible enzyme, no or poor activity in succinate- and octanoate-grown cells | Pseudomonas putida | - |
| 6.2.1.38 | culture condition:(+)-camphor-grown cell | inducible enzyme, no or poor activity in succinate- and octanoate-grown cells | Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.160 | 2.68 | - |
purified enzyme, pH 9.0, 30°C | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.160 | 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA + O2 + NADPH + H+ | specific substrate | Pseudomonas putida | delta-lactone of 5-hydroxy-3,4,4-trimethyl-A2-pimelyl-CoA + NADP+ + H2O | monooxygenase reaction, catalysis of a biological Baeyer-Villiger reaction with the formation of a lactone. This lactone product is unstable and susceptible to spontaneous ring opening | ? | |
| 1.14.13.160 | 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA + O2 + NADPH + H+ | specific substrate | Pseudomonas putida C1 / ATCC 17453 | delta-lactone of 5-hydroxy-3,4,4-trimethyl-A2-pimelyl-CoA + NADP+ + H2O | monooxygenase reaction, catalysis of a biological Baeyer-Villiger reaction with the formation of a lactone. This lactone product is unstable and susceptible to spontaneous ring opening | ? | |
| 1.14.13.160 | additional information | no substrate: 2,5-diketocamphane. Less than 5% of the activity with 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA is displayed with 2-oxo-DELTA3-4,5,5-trimethylcyclo-pentenylacetyl-CoA, 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA (hydrolyzed), 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetate-CoA, 2,5-diketocamphane | Pseudomonas putida | ? | - |
? | |
| 1.14.13.160 | additional information | no substrate: 2,5-diketocamphane. Less than 5% of the activity with 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA is displayed with 2-oxo-DELTA3-4,5,5-trimethylcyclo-pentenylacetyl-CoA, 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA (hydrolyzed), 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetate-CoA, 2,5-diketocamphane | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
| 1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas putida | [(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O | - |
? | |
| 1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas putida | [(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O | mass spectral analysis of the methyl ester of the product confirmes the insertion of a single oxygen atom | ? | |
| 1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas putida C1 / ATCC 17453 | [(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O | - |
? | |
| 1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas putida C1 / ATCC 17453 | [(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O | mass spectral analysis of the methyl ester of the product confirmes the insertion of a single oxygen atom | ? | |
| 6.2.1.38 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA | - |
Pseudomonas putida | AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA | - |
? | |
| 6.2.1.38 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate + ATP + CoA | - |
Pseudomonas putida C1 / ATCC 17453 | AMP + diphosphate + [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.160 | dimer | 2 * 56000, SDS-PAGE | Pseudomonas putida |
| 1.14.13.160 | homodimer | - |
Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.160 | 2-oxo-DELTA3-4,5,5-trimethylcyclopentenyl-acetyl-CoA monooxygenase | - |
Pseudomonas putida |
| 6.2.1.38 | 2-oxo-3-4,5,5-trimethylcyclopentenylacetyl CoA synthetase | - |
Pseudomonas putida |
| 6.2.1.38 | 2-oxo-3-4,5,5-trimethylcyclopentenylacetyl coenzyme A synthetase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.160 | 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.160 | 9 | - |
- |
Pseudomonas putida |
| 1.14.13.160 | 9 | - |
assay at | Pseudomonas putida |
| 6.2.1.38 | 8 | - |
- |
Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.160 | FAD | single molecule of FAD per dimer | Pseudomonas putida | |
| 1.14.13.160 | FAD | one molecule per enzyme molecule | Pseudomonas putida | |
| 1.14.13.160 | NADPH | specific for | Pseudomonas putida | |
| 1.14.13.160 | NADPH | specific for NADPH | Pseudomonas putida | |
| 6.2.1.38 | ATP | - |
Pseudomonas putida | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.13.160 | Pseudomonas putida | enzyme is induced by growth with (+)-camphor or 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid, but undetectable in extracts of octanoate- and succinate-grown cells | up |
| 1.14.13.160 | Pseudomonas putida | the enzyme is induced by cell growth on (+)-camphor as carbon source | up |
| 6.2.1.38 | Pseudomonas putida | the enzyme is induced by cell growth on (+)-camphor as carbon source | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.13.160 | metabolism | the enzyme catalyzes a step in the (+)-camphor catabolism. Metabolism of the first ring cleavage intermediate, 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid, occurs through the sequential action of two inducible enzymes, a coenzyme A ester synthetase, EC 6.2.1.38, and the monooxygenase | Pseudomonas putida |
| 6.2.1.38 | metabolism | the enzyme catalyzes a step in the (+)-camphor catabolism. Metabolism of the first ring cleavage intermediate, 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetic acid, occurs through the sequential action of two inducible enzymes, the coenzyme A ester synthetase and an oxygenase, EC 1.14.13.160 | Pseudomonas putida |
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