EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.99.46 | gene rutA, expression of the His-tagged enzyme in Escherichia coli strain BL21, co-expression with rutF | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.99.46 | purified recombinant RutA, X-ray diffraction structure determination and analysis at 1.8 A resolution | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.46 | thymine + FMNH2 + O2 | Escherichia coli | - |
(Z)-2-methylureidoacrylate peracid + FMN | - |
? | |
1.14.99.46 | uracil + FMNH2 + O2 | Escherichia coli | - |
(Z)-3-ureidoacrylate peracid + FMN | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.46 | Escherichia coli | P75898 | gene rutA, encoded in the b1012 operon, containing seven genes, rutA-G | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.99.46 | recombinant His-tagged RutA from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.46 | additional information | (Z)-3-ureidoacrylic peracid decomposes nonenzymatically to form primarily (Z)-3-ureidoacrylic acid and uracil, half life of (Z)-3-ureidoacrylic peracid is 3 hours at pH 10.0 at 25°C and much less at lower pH | Escherichia coli | ? | - |
? | |
1.14.99.46 | thymine + FMNH2 + O2 | - |
Escherichia coli | (Z)-2-methylureidoacrylate peracid + FMN | - |
? | |
1.14.99.46 | uracil + FMNH2 + O2 | - |
Escherichia coli | (Z)-3-ureidoacrylate peracid + FMN | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.99.46 | RutA | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.46 | FMN | - |
Escherichia coli | |
1.14.99.46 | additional information | RutA-catalyzed reduction or reduction by excess NADH or DTT in the reaction buffer | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.99.46 | metabolism | RutA is involved in the pyrimidine catabolic Rut pathway, which converts uracil to 3-hydroxypropionate or 2-methyl-3-hydroxypropionate. The flavoenzyme RutA, along with the flavin reductase RutF, catalyzes the initial uracil ring opening by an unprecedented oxidative hydrolysis reaction to give 3-ureidoacrylate | Escherichia coli |