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Literature summary extracted from
- Enhancement of the catalytic activity of a 27 kDa subtilisin-like enzyme from Bacillus amyloliquefaciens CH51 by in vitro mutagenesis (2011), J. Agric. Food Chem., 59, 8675-8682.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.62 | expressed in the protease deficient host Bacillus subtilis ISW1214 | Bacillus amyloliquefaciens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | G169A | mutant shows 1.4fold activity compared to wild-type | Bacillus amyloliquefaciens |
| 3.4.21.62 | S101K/G169A | mutant shows 3.3fold activity compared to wild-type | Bacillus amyloliquefaciens |
| 3.4.21.62 | S101L/G169A | mutant shows 2.9fold activity compared to wild-type | Bacillus amyloliquefaciens |
| 3.4.21.62 | S101R/G169A | mutant shows 2.2fold activity compared to wild-type | Bacillus amyloliquefaciens |
| 3.4.21.62 | S101W/G169A | mutant shows 3.9fold activity compared to wild-type | Bacillus amyloliquefaciens |
| 3.4.21.62 | S101W/G169A/V192A | mutant shows 1.4fold activity compared to wild-type, kcat doubled compared to wild-type, Km decreased compared to wild-type | Bacillus amyloliquefaciens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.62 | 0.15 | - |
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide | pH 7.4, 37°C, mutant S101W/G169A/V192A | Bacillus amyloliquefaciens |  |
| 3.4.21.62 | 0.35 | - |
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide | pH 7.4, 37°C, wild-type | Bacillus amyloliquefaciens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 27000 | - |
- |
Bacillus amyloliquefaciens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.62 | Bacillus amyloliquefaciens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.62 | using a HiTrap Q HP column | Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.62 | casein + H2O | caseinolytic activity is determined using the casein plate method | Bacillus amyloliquefaciens | ? | - |
? | |
| 3.4.21.62 | Human fibrinogen + H2O | fibrinolytic activity is determined using the fibrin plate method | Bacillus amyloliquefaciens | ? | - |
? | |
| 3.4.21.62 | N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Bacillus amyloliquefaciens | N-Succ-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | AprE51 | - |
Bacillus amyloliquefaciens |
| 3.4.21.62 | subtilisin A | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 37 | - |
assay at | Bacillus amyloliquefaciens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 45 | 50 | optimal reaction | Bacillus amyloliquefaciens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 50 | - |
remarkable loss of activity at temperature higher than 50°C | Bacillus amyloliquefaciens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.62 | 231.3 | - |
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide | pH 7.4, 37°C, wild-type | Bacillus amyloliquefaciens | |
| 3.4.21.62 | 586.2 | - |
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide | pH 7.4, 37°C, mutant S101W/G169A/V192A | Bacillus amyloliquefaciens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 7.4 | - |
assay at | Bacillus amyloliquefaciens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 7 | 11 | optimal reaction | Bacillus amyloliquefaciens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 4 | 12 | enzyme loses its activity at pH lower 4, retains 60% of its activity at pH higher 12 | Bacillus amyloliquefaciens |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.62 | 660 | - |
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide | pH 7.4, 37°C, wild-type | Bacillus amyloliquefaciens | |
| 3.4.21.62 | 3910 | - |
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide | pH 7.4, 37°C, mutant S101W/G169A/V192A | Bacillus amyloliquefaciens | |
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