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Literature summary extracted from

  • Dave, S.; Mahajan, S.; Chandra, V.; Gupta, P.
    Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point (2011), Int. J. Biol. Macromol., 49, 536-542.
    View publication on PubMed

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.32 2,2,2-trifluoroethanol the enhanced binding of 1-anilino-8-naphthalene sulfonic acid to the specific/pre-molten globule (SMG) state of stem bromelain upon addition of 30% 2,2,2-trifluoroethanol suggests the presence of a large number of solvent-accessible non-polar clusters in the treated SMG. The formation of an molten globe (MG)-like state characterized by disordered side chain interactions but with considerable secondary structure when the specific/pre-molten globule (SMG) state of stem bromelain is subjected to 30% 2,2,2-trifluoroethanol (TFE). The TFE-induced MG conformation at alkaline pH could represent the conformation that allows stem bromelain to traverse membranes Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 casein + H2O
-
 Ananas comosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.32 Sbm
-
 Ananas comosus
3.4.22.32 stem bromelain
-
 Ananas comosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.32 25
-
 assay at Ananas comosus