EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.63 | overexpression in Escherichia coli strain BL21(DE3) | Aspergillus fumigatus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.21.63 | additional information | an alp1 deletion strain, DELTAalp1, is generated from strain DELTAakuBKU80, and the culture supernatant possesses minimal complement-degrading activity. Moreover, protein extract derived from an Escherichia coli strain overproducing Alp1 cleaves C3b, C4b, and C5 | Aspergillus fumigatus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.21.63 | extracellular | secreted enzyme, large amounts in the culture supernatant | Aspergillus fumigatus | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.63 | additional information | Aspergillus fumigatus | secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins in culture supernatant C3, C4, C5, and C1q as well as immunoglobulin G. C4 is completely degraded. Degradation of C5 and C1q is also observed but is less prominent | ? | - |
? | |
3.4.21.63 | additional information | Aspergillus fumigatus DELTAakuBKU80 | secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins in culture supernatant C3, C4, C5, and C1q as well as immunoglobulin G. C4 is completely degraded. Degradation of C5 and C1q is also observed but is less prominent | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.63 | Aspergillus fumigatus | - |
- |
- |
3.4.21.63 | Aspergillus fumigatus DELTAakuBKU80 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.63 | additional information | secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins in culture supernatant C3, C4, C5, and C1q as well as immunoglobulin G. C4 is completely degraded. Degradation of C5 and C1q is also observed but is less prominent | Aspergillus fumigatus | ? | - |
? | |
3.4.21.63 | additional information | secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins in culture supernatant C3, C4, C5, and C1q as well as immunoglobulin G. C4 is completely degraded. Degradation of C5 and C1q is also observed but is less prominent | Aspergillus fumigatus DELTAakuBKU80 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.63 | protease Alp1 | - |
Aspergillus fumigatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 37 | - |
assay at | Aspergillus fumigatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 7.5 | - |
assay at | Aspergillus fumigatus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.21.63 | physiological function | secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins C3, C4, and C5. C3b is an important opsonin, but other complement proteins also have essential functions in the defense against microbes. C5b, a cleavage product of C5, initiates formation of the membrane attack complex at a later stage of complement activation, and C5a represents a potent anaphylatoxin. C1q initiates the classical pathway of complement activation, and C4 is part of the C3 convertase of the classical pathway. The enzyme is involved in the mechanism that contributes to evasion from the host complement attack, overview | Aspergillus fumigatus |