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Literature summary extracted from

  • Larsen, T.; Petersen, B.O.; Storgaard, B.G.; Duus, J.O.; Palcic, M.M.; Leisner, J.J.
    Characterization of a novel Salmonella typhimurium chitinase which hydrolyzes chitin, chitooligosaccharides and an N-acetyllactosamine conjugate (2011), Glycobiology, 21, 426-436.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 a C-terminal truncated form of chiA lacking a putative chitin-binding domain, recombinant expression of the N-terminally His-tagged mutant in Escherichia coli strain BL21 (DE3) Salmonella enterica typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 71365
-
 x * 71365, truncated ChiA mutant enzyme ChiADELTACtBM, sequence calculation Salmonella enterica typhimurium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Salmonella enterica typhimurium
-
 gene SL0018 or chiA
-
3.2.1.14 Salmonella enterica typhimurium SL1344
-
 gene SL0018 or chiA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
 Salmonella enterica typhimurium ?
-
 ?
3.2.1.14 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
 Salmonella enterica typhimurium SL1344 ?
-
 ?
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Salmonella enterica typhimurium ?
-
 ?
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Salmonella enterica typhimurium SL1344 ?
-
 ?
3.2.1.14 carboxymethyl-Chitin-Remazol-Brilliant-Violet + H2O
-
 Salmonella enterica typhimurium ?
-
 ?
3.2.1.14 carboxymethyl-Chitin-Remazol-Brilliant-Violet + H2O
-
 Salmonella enterica typhimurium SL1344 ?
-
 ?
3.2.1.14 chitin + H2O
-
 Salmonella enterica typhimurium N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.14 chitin + H2O
-
 Salmonella enterica typhimurium SL1344 N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.14 additional information the purified enzyme hydrolyzes 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside, and carboxymethyl-Chitin-Remazol-Brilliant-Violet, but does not act on 4-nitrophenyl N-acetyl-beta-D-glucosaminide, peptidoglycan or 4-nitrophenyl beta-D-cellobioside. Hydrolysis occurs with the retention of configuration and the enzyme acts on only the beta-anomers of chitooligosaccharide substrates. The enzyme also released N-acetyllactosamine disaccharide from Galbeta1,4GlcNAcbeta-O-(CH2)8CONH(CH2)2NHCO-tetramethylrhodamine, a model substrate for LacNAc terminating glycoproteins and glycolipids Salmonella enterica typhimurium ?
-
 ?
3.2.1.14 additional information the purified enzyme hydrolyzes 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside, and carboxymethyl-Chitin-Remazol-Brilliant-Violet, but does not act on 4-nitrophenyl N-acetyl-beta-D-glucosaminide, peptidoglycan or 4-nitrophenyl beta-D-cellobioside. Hydrolysis occurs with the retention of configuration and the enzyme acts on only the beta-anomers of chitooligosaccharide substrates. The enzyme also released N-acetyllactosamine disaccharide from Galbeta1,4GlcNAcbeta-O-(CH2)8CONH(CH2)2NHCO-tetramethylrhodamine, a model substrate for LacNAc terminating glycoproteins and glycolipids Salmonella enterica typhimurium SL1344 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 71365, truncated ChiA mutant enzyme ChiADELTACtBM, sequence calculation Salmonella enterica typhimurium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiA
-
 Salmonella enterica typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 37
-
-
 Salmonella enterica typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6.1
-
-
 Salmonella enterica typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 7.5 high activity in this range Salmonella enterica typhimurium