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Literature summary extracted from

  • Sun, L.; Zhu, B.; Wu, H.; Yu, L.; Zhou, D.; Dong, X.; Yang, J.; Li, D.; Ye, W.; Murata, Y.
    Purification and characterization of cathepsin B from the gut of the sea cucumber (Stichopus japonicas) (2011), Food Sci. Biotechnol., 20, 919-925.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 1,10-phenanthroline 77.3% residual activity at 1 mM Apostichopus japonicus
3.4.22.1 antipain complete inhibition at 1 mM Apostichopus japonicus
3.4.22.1 Cu2+ 32% residual activity at 1 mM Apostichopus japonicus
3.4.22.1 E-64 complete inhibition at 1 mM Apostichopus japonicus
3.4.22.1 Fe2+ 79.9% residual activity at 1 mM Apostichopus japonicus
3.4.22.1 Fe3+ 82.3% residual activity at 1 mM Apostichopus japonicus
3.4.22.1 iodoacetic acid complete inhibition at 1 mM Apostichopus japonicus
3.4.22.1 leupeptin 42.5% residual activity at 0.1 mM Apostichopus japonicus
3.4.22.1 Mn2+ 85.4% residual activity at 1 mM Apostichopus japonicus
3.4.22.1 additional information not inhibited by dithiothreitol, L-cysteine, EDTA, and EGTA Apostichopus japonicus
3.4.22.1 Ni2+ 26.7% residual activity at 1 mM Apostichopus japonicus
3.4.22.1 phenylmethylsulfonyl fluoride 62.8% residual activity at 1 mM Apostichopus japonicus
3.4.22.1 Soybean trypsin inhibitor 51.2% residual activity at 0.1 g/l Apostichopus japonicus
3.4.22.1 Zn2+ 14.7% residual activity at 1 mM Apostichopus japonicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.1 Ca2+ a slight increase of activity (4.2%) is induced by 1 mM Ca2+ Apostichopus japonicus
3.4.22.1 Mg2+ a slight increase of activity (3.3%) is induced by 1 mM Mg2+ Apostichopus japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 23000
-
 1 * 26000 + 1 * 23000, SDS-PAGE Apostichopus japonicus
3.4.22.1 26000
-
 1 * 26000 + 1 * 23000, SDS-PAGE Apostichopus japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Apostichopus japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1 ammonium sulfate fractionation, DEAE Sepharose CL-6B column chromatography, Sephadex G-75 gel filtration, and TSK-Gel 3000 SWxl gel filtration Apostichopus japonicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 gut
-
 Apostichopus japonicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.1 1.19
-
 crude extract, at pH 5.5 and 45°C Apostichopus japonicus
3.4.22.1 96.7
-
 after 81.26fold purification, at pH 5.5 and 45°C Apostichopus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Apostichopus japonicus N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 N-carbobenzoxy-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Apostichopus japonicus N-carbobenzoxy-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.1 heterodimer 1 * 26000 + 1 * 23000, SDS-PAGE Apostichopus japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.1 45
-
-
 Apostichopus japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.1 50
-
 about 80% activity after 10 min at 45°C, the enzyme activity remains stable for 20 min at 45°C, and decreases with the incubation time extending to 70 min, with a 50% activity loss Apostichopus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 5.5
-
-
 Apostichopus japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.1 4.5 6 the protease activity is stable in the acidic range between pH 4.5 and 6.0, and declines above pH 6.5 rapidly. At pH 6.5, more than 60% of the enzyme activity is lost Apostichopus japonicus