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Literature summary extracted from

  • Liu, C.H.; Shiu, Y.L.; Hsu, J.L.
    Purification and characterization of trypsin from the pyloric ceca of orange-spotted grouper, Epinephelus coioides (2011), Fish Physiol. Biochem., 38, 837-848.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane 16.9% inhibition at 0.1 mM Epinephelus coioides
3.4.21.4 EDTA 14.2% inhibition at 2 mM Epinephelus coioides
3.4.21.4 iodoacetic acid 16.5% inhibition at 1 mM; 1 mM, 16.5% inhibition Epinephelus coioides
3.4.21.4 additional information no inhibition by N-ethylmaleimide Epinephelus coioides
3.4.21.4 N-p-tosyl-L-lysine chloromethyl ketone 5 mM, 83% inhibition Epinephelus coioides
3.4.21.4 N-tosyl-L-lysine chloromethyl ketone 82.9% inhibition at 5 mM Epinephelus coioides
3.4.21.4 NaCl 69% inhibition at 0.6 M Epinephelus coioides
3.4.21.4 pepstatin A 26% inhibition at 0.01 mM Epinephelus coioides
3.4.21.4 Soybean trypsin inhibitor 1 mg/ml, 80% inhibition; 80.3% inhibition at 1 mg/ml Epinephelus coioides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 24000
-
 x * 24000, SDS-PAGE Epinephelus coioides

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Epinephelus coioides
-
-
-
3.4.21.4 Epinephelus coioides G9JKF3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4
-
 Epinephelus coioides
3.4.21.4 native enzyme 161.85fold by ammonium sulfate, ionic exchange chromatography, ultrafiltratrion, and benzamidine affinity chromatography Epinephelus coioides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 cecum pyloric Epinephelus coioides
-
3.4.21.4 intestine anterior, middle, and hind Epinephelus coioides
-
3.4.21.4 pyloric cecum
-
 Epinephelus coioides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.4 43.24
-
 purified enzyme, pH 8.0, 37°C Epinephelus coioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 Nalpha-benzoyl-L-arginine ethyl ester + H2O
-
 Epinephelus coioides Nalpha-benzoyl-L-arginine + ethanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.4 ? x * 24000, SDS-PAGE Epinephelus coioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.4 50
-
-
 Epinephelus coioides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.4 20 70 40% of maximal activity at 20°C, 30% at 70°C Epinephelus coioides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.4 20 50 purified enzyme, stable Epinephelus coioides
3.4.21.4 60
-
 purified enzyme, 52.63% of maximal activity remaining Epinephelus coioides
3.4.21.4 70
-
 purified enzyme, 22.39% of maximal activity remaining Epinephelus coioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 8 10
-
 Epinephelus coioides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.4 4
-
 purified enzyme, 53.55% of maximal activity remaining Epinephelus coioides
3.4.21.4 6 8 purified enzyme, stable Epinephelus coioides
3.4.21.4 11
-
 purified enzyme, 66.25% of maximal activity remaining Epinephelus coioides