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Literature summary extracted from

  • Sauer, K.; Thauer, R.K.
    Methanol:coenzyme M methyltransferase from Methanosarcina barkeri - identification of the active-site histidine in the corrinoid-harboring subunit MtaC by site-directed mutagenesis (1998), Eur. J. Biochem., 253, 698-705.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.246 Ti(III) citrate
-
 Methanosarcina barkeri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.246 genes mtaA and mtaB, expression of N-terminally His-tagged isozymes MtaA and MtbA in Escherichia coli strain M15 Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.246 Zn2+ required Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.246 a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M Methanosarcina barkeri
-
 methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.246 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.246 recombinant N-terminally His-tagged isozymes MtaA and MtbA from Escherichia coli strain M15 by nickel affinity chromatography Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.246 a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
 ?
2.1.1.246 additional information recombinant MtaA catalyzes the formation of methyl-coenzyme M from free CH3-cob(III)alamin and coenzyme M at specific rates comparable to those predicted for MtaA-catalyzed MtaC demethylation Methanosarcina barkeri ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.246 methanol:coenzyme M methyltransferase
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.246 37
-
 assay at Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.246 7
-
 assay at Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.246 evolution Methanosarcina barkeri contains an isoenzyme of MtaA designated MtbA. The isoenzyme reacts with MtaC with only 2.5% of the activity of MtaA Methanosarcina barkeri
2.1.1.246 metabolism the enzyme system catalyzing the formation of methyl-coenzyme M from methanol and coenzyme M in Methanosarcina barkeri is composed of the three different polypeptides MtaA, MtaB and MtaC of which MtaC harbors a corrinoid prosthetic group Methanosarcina barkeri
2.1.1.246 additional information the enzyme system catalyzing the formation of methyl-coenzyme M from methanol and coenzyme M in Methanosarcina barkeri is composed of the three different polypeptides MtaA, MtaB and MtaC of which MtaC harbors a corrinoid prosthetic group Methanosarcina barkeri