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Literature summary extracted from

  • Wassenaar, R.; Keltjens, J.; Van Der Drift, C.; Vogels, G.
    Purification and characterization of dimethylamine: 5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro (1998), Eur. J. Biochem., 253, 692-697.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.249 ATP acts as a powerful allosteric effector on the methyltransferase reaction. ATP serves as the substrate for MAP, which, after being phosphorylated by ATP, operates in the reductive activation of the corrinoid methyltransferase Methanosarcina barkeri
2.1.1.249 methyltransferase activating protein MAP, dependent on. ATP serves as the substrate for MAP, which, after being phosphorylated by ATP, operates in the reductive activation of the corrinoid methyltransferase Methanosarcina barkeri
2.1.1.249 additional information as isolated, DMA-MT is inactive, but it can enzymically be reactivated by methyltransferase activating protein, ATP, and hydrogenase Methanosarcina barkeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.249 Monomethylamine competitive inhibitor Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.249 50000
-
 2 * 50000, SDS-PAGE Methanosarcina barkeri
2.1.1.249 100000
-
 gel filtration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] Methanosarcina barkeri Fusaro / DSM 804
-
 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.249 Methanosarcina barkeri
-
 DSM 804
-
2.1.1.249 Methanosarcina barkeri Fusaro / DSM 804
-
 DSM 804
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.249 native enzyme 62.8fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and anion exchange chromatography Methanosarcina barkeri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.249 culture condition:trimethylamine-grown cell
-
 Methanosarcina barkeri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] methylation of the corrinoid prosthetic group Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri Fusaro / DSM 804 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] methylation of the corrinoid prosthetic group Methanosarcina barkeri Fusaro / DSM 804 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.249 homodimer 2 * 50000, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.249 dimethyl-12-HBI methyltransferase
-
 Methanosarcina barkeri
2.1.1.249 dimethylamine/5-hydroxybenzimidazolylcobamide methyltransferase
-
 Methanosarcina barkeri
2.1.1.249 DMA-MT
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.249 46
-
-
 Methanosarcina barkeri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.249 20 46 rapid decline of activity above 46°C, inactive at 55°C Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.249 6.9
-
-
 Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.249 corrinoid protein prosthetic group, with a corrinoid content of 0.9 mol B12/mol holoenzyme Methanosarcina barkeri

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.249 4.5
-
 Monomethylamine pH 7.0, 37°C Methanosarcina barkeri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.249 Methanosarcina barkeri isoelectric focusing
-
 4.7

General Information

EC Number General Information Comment Organism
2.1.1.249 additional information as-isolated DMA-MT is inactive. The enzyme can be reactivated to yield the methylated B12-HBI prosthetic group by a system that comprises a methyl donor, a reductant, ATP and one (or more) component(s) present in the partially purified methyltransferase activating protein, MAP, fraction Methanosarcina barkeri