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Literature summary extracted from

  • Galdino, A.S.; Silva, R.N.; Lottermann, M.T.; Alvares, A.C.; de Moraes, L.M.; Torres, F.A.; de Freitas, S.M.; Ulhoa, C.J.
    Biochemical and structural characterization of Amy1: an alpha-amylase from Cryptococcus flavus expressed in Saccharomyces cerevisiae (2011), Enzyme Res., 2011, 157294.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Sacharomyces cerevisiae strain CENPK2 Saitozyma flava

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Cu2+ complete inhibition at 4 mM Saitozyma flava
3.2.1.1 Hg2+ complete inhibition at 4 mM Saitozyma flava
3.2.1.1 Mg2+ 11% inhibition at 4 mM Saitozyma flava
3.2.1.1 Mn2+ 22% inhibition at 4 mM Saitozyma flava
3.2.1.1 Zn2+ 80% inhibition at 4 mM Saitozyma flava

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
 Saitozyma flava
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 NH4+ activates Saitozyma flava

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 67000
-
 x * 67000, SDS-PAGE Saitozyma flava

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Saitozyma flava
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant enzyme 3.8fold from Saccharomyces cerevisiae by anion exchange chromatography and gel filtration to homogeneity Saitozyma flava

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 4.16
-
 purified recombinant enzyme, pH 5.5, 60°C Saitozyma flava

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O
-
 Saitozyma flava ?
-
 ?
3.2.1.1 amylose + H2O
-
 Saitozyma flava ?
-
 ?
3.2.1.1 glycogen + H2O
-
 Saitozyma flava ?
-
 ?
3.2.1.1 additional information activity of Amy1 towards soluble starch, glycogen, amylopectin, amylose in descending order Saitozyma flava ?
-
 ?
3.2.1.1 soluble starch + H2O
-
 Saitozyma flava ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 67000, SDS-PAGE Saitozyma flava

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 AMY1
-
 Saitozyma flava

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 60
-
-
 Saitozyma flava

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 30 65 activity range, profile, overview Saitozyma flava

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 55
-
 purified recombinant enzyme, 1 h, 90% activity remaining Saitozyma flava
3.2.1.1 60
-
 purified recombinant enzyme, 1 h, 50% activity remaining Saitozyma flava

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5.5
-
-
 Saitozyma flava

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 1 8 activity range, profile, overview Saitozyma flava

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 3 6 over 80% acivity remaining within this range Saitozyma flava