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Literature summary extracted from

  • Ryu, H.; Jin, X.; Lee, J.; Woo, H.; Kim, Y.; Kim, G.; Seo, E.; Kang, H.; Kim, J.; Cho, D.; Kimura, A.; Kim, D.
    Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities (2010), Enzyme Microb. Technol., 47, 212-215.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 gene dsxr, the recombinant chimeric dsxr encodes dextranase-dextransucrase, DXSR, is sequentially composed of the dextransucrase-encoding gene dsrBCB4 and the gene dex2, encoding the dextranase from Arthrobacter oxidans, UniProt ID A9UKG4, expression in Escherichia coli strain BL21(DE3)pLysS Leuconostoc mesenteroides
3.2.1.11 gene dex2, expression of the engineered fusion enzyme DSXR in Escherichia coli strain BL21(DE3)pLysS, optimization of protein expression conditions for enhancement of protein production by the effects of three-level-three-factors and their mutual interaction in Escherichia coli Pseudarthrobacter oxydans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.5 additional information construction and optimal expression of a fusion enzyme DSXR having dextransucrase and dextranase activities, for optimization of protein expression, response surface methodology is used Leuconostoc mesenteroides
3.2.1.11 additional information generation of an engineered fusion enzyme of dextransucrase, from dsrBCB4 gene from Leuconostoc mesenteroides B-1299CB4, and Arthrobacter oxydans dextranase, i.e. DSXR. DSXR is potentially useful for reliable production of long isomalto-oligosaccharides from sucrose by a one-step reaction Pseudarthrobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides D2CFL0 gene dsrBCB4
-
3.2.1.11 Pseudarthrobacter oxydans A9UKG4 gene dex2
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 additional information
-
 120.1 U/ml, recombinant dextransucrase activity of recombinant DSXR chimeric enzyme, pH 5.8, 28°C Leuconostoc mesenteroides
2.4.1.5 1.2
-
 purified recombinant chimeric DSXR, dextransucrase activity, pH 5.8, 28°C Leuconostoc mesenteroides
3.2.1.11 additional information
-
 activities of recombinant fusion protein and of wild-type dextranase in fermentation, overview Pseudarthrobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 28
-
 assay at Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.2
-
-
 Leuconostoc mesenteroides
3.2.1.11 5.2
-
 recombinant wild-type dextranase Pseudarthrobacter oxydans
3.2.1.11 5.8
-
 recombinant engineered fusion protein DSXR Pseudarthrobacter oxydans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.5 5 6.4 highly reduced activity below pH 5.0 and above pH 6.4 Leuconostoc mesenteroides
3.2.1.11 5 6.4 great loss in activity below and above that values, recombinant wild-type dextranase and engineered fusion protein DSXR Pseudarthrobacter oxydans