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Literature summary extracted from

  • Liu, D.; Cai, J.; Xie, C.; Liu, C.; Chen, Y.
    Purification and partial characterization of a 36-kDa chitinase from Bacillus thuringiensis subsp. colmeri, and its biocontrol potential (2010), Enzyme Microb. Technol., 46, 252-256.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.14 EDTA
-
 Bacillus thuringiensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chiA, expression in Escherichia coli strain XL-1 Blue Bacillus thuringiensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ag+ inhibits 57.2% at 10 mM Bacillus thuringiensis
3.2.1.14 Cr3+ inhibits 12.3% at 10 mM Bacillus thuringiensis
3.2.1.14 Mn2+ inhibits 7.6% at 10 mM Bacillus thuringiensis
3.2.1.14 SDS inhibits 9.1% at 10 mM Bacillus thuringiensis
3.2.1.14 Zn2+ inhibits 63.8% at 10 mM Bacillus thuringiensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Cu2+ activates 15% at 10 mM Bacillus thuringiensis
3.2.1.14 Fe3+ activates 6% at 10 mM Bacillus thuringiensis
3.2.1.14 Mg2+ activates 6% at 10 mM Bacillus thuringiensis
3.2.1.14 Na+ activates 6% at 10 mM Bacillus thuringiensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 36000
-
 2 * 36000, ChiA, SDS-PAGE Bacillus thuringiensis
3.2.1.14 72000
-
 native PAGE Bacillus thuringiensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus thuringiensis
-
 subsp. colmeri
-
3.2.1.14 Bacillus thuringiensis 15A3
-
 subsp. colmeri
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 extracellular enzyme by gel filtration Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin
-
 Bacillus thuringiensis ?
-
 ?
3.2.1.14 colloidal chitin
-
 Bacillus thuringiensis 15A3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 dimer 2 * 36000, ChiA, SDS-PAGE Bacillus thuringiensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiA
-
 Bacillus thuringiensis
3.2.1.14 chitinase A
-
 Bacillus thuringiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Bacillus thuringiensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 20 60 activity range Bacillus thuringiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Bacillus thuringiensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 8 activity range Bacillus thuringiensis

General Information

EC Number General Information Comment Organism
3.2.1.14 physiological function ChiA significantly inhibits the spore germination of four species of fungi. The IC50 of ChiA on the spore germination of Penicillium glaucum and Sclerotinia fuckelian are 0.0113 mg/ml and 0.0106 mg/ml, respectively. The crude ChiA protein at 12.6 mU reduced the LC50 of the crystal protein of strain 15A3 against the larvae of Spodoptera exigua and Helicoverpa armigera Bacillus thuringiensis