EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.23.48 | expression in Escherichia coli | Yersinia pestis |
3.4.23.49 | expression in Escherichia coli | Erwinia pyrifoliae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.23.48 | comparison of the structure of Pla and the modeled structure of protease Epo of the plant pathogenic Erwinia pyrifoliae | Yersinia pestis |
3.4.23.49 | comparison of the structure of Pla and the modeled structure of protease Epo of the plant pathogenic Erwinia pyrifoliae | Erwinia pyrifoliae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.23.48 | additional information | swapping of ten amino acid residues at two surface loops of Pla and membrane protease Epo of the plant pathogenic Erwinia pyrifoliae introduces plasminogen activation capacity in Epo and inactivates the function in Pla | Yersinia pestis |
3.4.23.49 | additional information | swapping of ten amino acid residues at two surface loops of Pla and membrane protease Epo of the plant pathogenic Erwinia pyrifoliae introduces plasminogen activation capacity in Epo and inactivates the function in Pla | Erwinia pyrifoliae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.23.48 | Yersinia pestis | P17811 | - |
- |
3.4.23.49 | Erwinia pyrifoliae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.23.48 | alpha2-antiplasmin + H2O | a serpin | Yersinia pestis | ? | - |
? | |
3.4.23.48 | PAI-1 + H2O | a serpin | Yersinia pestis | ? | - |
? | |
3.4.23.48 | plasminogen + H2O | - |
Yersinia pestis | plasmin + ? | - |
? | |
3.4.23.49 | alpha2-antiplasmin + H2O | a serpin | Erwinia pyrifoliae | ? | - |
? | |
3.4.23.49 | PAI-1 + H2O | a serpin | Erwinia pyrifoliae | ? | - |
? | |
3.4.23.49 | plasminogen + H2O | poor substrate | Erwinia pyrifoliae | ? | no formation of plasmin light chain | ? |