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Literature summary extracted from

  • Haiko, J.; Laakkonen, L.; Westerlund-Wikstroem, B.; Korhonen, T.K.
    Molecular adaptation of a plant-bacterium outer membrane protease towards plague virulence factor Pla (2011), BMC Evol. Biol., 11, 43.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.48 expression in Escherichia coli Yersinia pestis
3.4.23.49 expression in Escherichia coli Erwinia pyrifoliae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.23.48 comparison of the structure of Pla and the modeled structure of protease Epo of the plant pathogenic Erwinia pyrifoliae Yersinia pestis
3.4.23.49 comparison of the structure of Pla and the modeled structure of protease Epo of the plant pathogenic Erwinia pyrifoliae Erwinia pyrifoliae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.48 additional information swapping of ten amino acid residues at two surface loops of Pla and membrane protease Epo of the plant pathogenic Erwinia pyrifoliae introduces plasminogen activation capacity in Epo and inactivates the function in Pla Yersinia pestis
3.4.23.49 additional information swapping of ten amino acid residues at two surface loops of Pla and membrane protease Epo of the plant pathogenic Erwinia pyrifoliae introduces plasminogen activation capacity in Epo and inactivates the function in Pla Erwinia pyrifoliae

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.48 Yersinia pestis P17811
-
-
3.4.23.49 Erwinia pyrifoliae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.48 alpha2-antiplasmin + H2O a serpin Yersinia pestis ?
-
?
3.4.23.48 PAI-1 + H2O a serpin Yersinia pestis ?
-
?
3.4.23.48 plasminogen + H2O
-
Yersinia pestis plasmin + ?
-
?
3.4.23.49 alpha2-antiplasmin + H2O a serpin Erwinia pyrifoliae ?
-
?
3.4.23.49 PAI-1 + H2O a serpin Erwinia pyrifoliae ?
-
?
3.4.23.49 plasminogen + H2O poor substrate Erwinia pyrifoliae ? no formation of plasmin light chain ?