Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cadavid-Restrepo, G.; Gastardelo, T.; Faudry, E.; De Almeida, H.; Bastos, I.; Negreiros, R.; Lima, M.; Assump?ß?£o, T.; Almeida, K.; Ragno, M.; Ebel, C.; Ribeiro, B.; Felix, C.; Santana, J.
    The major leucyl aminopeptidase of Trypanosoma cruzi (LAPTc) assembles into a homohexamer and belongs to the M17 family of metallopeptidases (2011), BMC Biochem., 12, 46.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 His-tagged protein expressed in Escherichia coli BL21(DE3) Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline 0.25 mM: 17% activity, restored by Mn2+ and Ca2+ Trypanosoma cruzi
3.4.11.1 bestatin 0.1 mM: 3% activity, activity Trypanosoma cruzi
3.4.11.1 EDTA 10 mM: 55% activity, restored by Mn2+ and Ca2+ Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.012
-
 L-Leu-7-amido-4-methylcoumarin native enzyme, pH 7.5, 37°C Trypanosoma cruzi
3.4.11.1 0.1859
-
 L-Leu-7-amido-4-methylcoumarin recombinant enzyme, pH 7.5, 37°C Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 55000
-
 6 * 55000, SDS-PAGE, calculated: 55891 Da Trypanosoma cruzi
3.4.11.1 320000
-
 gel filtration Trypanosoma cruzi
3.4.11.1 330000
-
 analytical ultracentrifugation Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Trypanosoma cruzi Q4DZJ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 ion exchange chromatography (DEAE), gel filtration; His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), gel filtration Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi L-Leu + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 homohexamer 6 * 55000, SDS-PAGE, calculated: 55891 Da Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 major leucyl aminopeptidase metalloaminopeptidase Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 native enzyme Trypanosoma cruzi
3.4.11.1 60
-
 recombinant enzyme, 25% activity at 37°C Trypanosoma cruzi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 additional information
-
 recombinant enzyme is more stable than native enzyme: after 15 min at 60°C native form has 35% and recombinant form 87% activity Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 12.47
-
 L-Leu-7-amido-4-methylcoumarin native enzyme, pH 7.5, 37°C Trypanosoma cruzi
3.4.11.1 34.84
-
 L-Leu-7-amido-4-methylcoumarin recombinant enzyme, pH 7.5, 37°C Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 7
-
 native enzyme Trypanosoma cruzi
3.4.11.1 8
-
 recombinant enzyme Trypanosoma cruzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 6 8 native enzyme, 45% activity at pH 6.0 and 8.0, inactive at pH 5.0 and 9.0 Trypanosoma cruzi
3.4.11.1 7.5 9 recombinant enzyme, 40% activity at pH 7.5, 75% activity at pH 9.0 Trypanosoma cruzi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.1 190
-
 L-Leu-7-amido-4-methylcoumarin recombinant enzyme, pH 7.5, 37°C Trypanosoma cruzi
3.4.11.1 1040
-
 L-Leu-7-amido-4-methylcoumarin native enzyme, pH 7.5, 37°C Trypanosoma cruzi