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Literature summary extracted from

  • Maruyama, R.; Nishizawa, M.; Itoi, Y.; Ito, S.; Inoue, M.
    Isolation and expression of a Bacillus cereus gene encoding benzil reductase (2001), Biotechnol. Bioeng., 75, 630-633.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.320 gene yueD, Bacillus cereus library screening in Escherichia coli and cloning of the gene encoding the benzil reductase, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain DH5alpha Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.320 (S)-benzoin + NADP+ Bacillus cereus stereospecific asymmetric reduction of benzil to (S)-benzoin benzil + NADPH + H+
-
 r
1.1.1.320 (S)-benzoin + NADP+ Bacillus cereus Tim-r01 stereospecific asymmetric reduction of benzil to (S)-benzoin benzil + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.320 Bacillus cereus Q8RJB2
-
-
1.1.1.320 Bacillus cereus Tim-r01 Q8RJB2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.320 (S)-benzoin + NADP+ stereospecific asymmetric reduction of benzil to (S)-benzoin Bacillus cereus benzil + NADPH + H+
-
 r
1.1.1.320 (S)-benzoin + NADP+ stereospecific asymmetric reduction of benzil to (S)-benzoin Bacillus cereus Tim-r01 benzil + NADPH + H+
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.320 NADP+
-
 Bacillus cereus
1.1.1.320 NADPH
-
 Bacillus cereus

General Information

EC Number General Information Comment Organism
1.1.1.320 evolution the enzyme is a member of the short-chain dehydrogenase/reductase, SDR, family Bacillus cereus