EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | DMSO | stimulates at 1 mM | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Urea | stimulates at 1 mM | Saccharopolyspora pathumthaniensis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.8 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Saccharopolyspora pathumthaniensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | Ba2+ | - |
Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Cu2+ | slight inhibition | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | EDTA | slight inhibition | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Fe2+ | slight inhibition | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Hg2+ | complete inhibition | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | iodoacetic acid | - |
Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Na+ | slight inhibition | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Ni2+ | slight inhibition | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | SDS | - |
Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Zn2+ | slight inhibition | Saccharopolyspora pathumthaniensis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.8 | extracellular | - |
Saccharopolyspora pathumthaniensis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | Cs+ | stimulates | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | K+ | stimulates slightly | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Rb+ | stimulates | Saccharopolyspora pathumthaniensis | |
3.2.1.8 | Sn2+ | stimulates slightly | Saccharopolyspora pathumthaniensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 36000 | - |
x * 36000, SDS-PAGE | Saccharopolyspora pathumthaniensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Saccharopolyspora pathumthaniensis | E1APH5 | isolated from the gut of a grass-feeding termite | - |
3.2.1.8 | Saccharopolyspora pathumthaniensis S582 | E1APH5 | isolated from the gut of a grass-feeding termite | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | native extracellular enzyme 167fold to homogeneity | Saccharopolyspora pathumthaniensis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | additional information | hydrolysis of best substrate beechwood xylan by the enzyme results in formation of xylobiose and xylose as major products. The enzyme is specific for xylan acting as an endo-type xylanase. Soluble oat spelt xylan is also a good substrate, while insoluble oat spelt xylan is not. No activity with Avicel, 4-nitrophenyl beta-D-xylopyranoside, and locust bean gum | Saccharopolyspora pathumthaniensis | ? | - |
? | |
3.2.1.8 | additional information | hydrolysis of best substrate beechwood xylan by the enzyme results in formation of xylobiose and xylose as major products. The enzyme is specific for xylan acting as an endo-type xylanase. Soluble oat spelt xylan is also a good substrate, while insoluble oat spelt xylan is not. No activity with Avicel, 4-nitrophenyl beta-D-xylopyranoside, and locust bean gum | Saccharopolyspora pathumthaniensis S582 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.8 | ? | x * 36000, SDS-PAGE | Saccharopolyspora pathumthaniensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | More | the enzyme is a member of the G10 glucohydrolase family | Saccharopolyspora pathumthaniensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 70 | - |
- |
Saccharopolyspora pathumthaniensis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 40 | 80 | activity range, profile, overview | Saccharopolyspora pathumthaniensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 50 | - |
purified enzyme, 2 h, stable | Saccharopolyspora pathumthaniensis |
3.2.1.8 | 60 | - |
purified enzyme, half-life is 180 min | Saccharopolyspora pathumthaniensis |
3.2.1.8 | 70 | - |
purified enzyme, half-life is 120 min | Saccharopolyspora pathumthaniensis |
3.2.1.8 | 80 | - |
purified enzyme, inactivation | Saccharopolyspora pathumthaniensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6.5 | - |
- |
Saccharopolyspora pathumthaniensis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 5 | 10 | activity range, profile, overview | Saccharopolyspora pathumthaniensis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 5.5 | 10 | purified enzyme, stable | Saccharopolyspora pathumthaniensis |