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Literature summary extracted from

  • Rastogi, G.; Bhalla, A.; Adhikari, A.; Bischoff, K.M.; Hughes, S.R.; Christopher, L.P.; Sani, R.K.
    Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains (2010), Biores. Technol., 101, 8798-8806.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 1.08
-
 carboxymethyl cellulose pH 5.0, 70°C Geobacillus sp.
3.2.1.4 3.11
-
 carboxymethyl cellulose pH 5.0, 75°C Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O Geobacillus sp.
-
 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O Bacillus sp. (in: Bacteria) DUSELR13
-
 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O Geobacillus sp. WSUCFI
-
 ?
-
 ?
3.2.1.4 cellulose + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
3.2.1.4 cellulose + H2O Geobacillus sp.
-
 ?
-
 ?
3.2.1.4 cellulose + H2O Bacillus sp. (in: Bacteria) DUSELR13
-
 ?
-
 ?
3.2.1.4 cellulose + H2O Geobacillus sp. WSUCFI
-
 ?
-
 ?
3.2.1.4 additional information Bacillus sp. (in: Bacteria) the organism possesses carboxymethyl cellulase and cellulase activities, overview ?
-
 ?
3.2.1.4 additional information Geobacillus sp. the organism possesses carboxymethyl cellulase and cellulase activities, overview ?
-
 ?
3.2.1.4 additional information Bacillus sp. (in: Bacteria) DUSELR13 the organism possesses carboxymethyl cellulase and cellulase activities, overview ?
-
 ?
3.2.1.4 additional information Geobacillus sp. WSUCFI the organism possesses carboxymethyl cellulase and cellulase activities, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Bacillus sp. (in: Bacteria)
-
 isolated from a cellulose-degrading enrichment culture initiated from compost samples
-
3.2.1.4 Bacillus sp. (in: Bacteria) DUSELR13
-
 isolated from a cellulose-degrading enrichment culture initiated from compost samples
-
3.2.1.4 Geobacillus sp.
-
 isolated from a cellulose-degrading enrichment culture initiated from compost samples
-
3.2.1.4 Geobacillus sp. WSUCFI
-
 isolated from a cellulose-degrading enrichment culture initiated from compost samples
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 additional information the optimum growth medium pH is 6.0 for maximum production of CMCase in DUSEL R13, and best carbon and energy source is cellulose, overview Bacillus sp. (in: Bacteria)
-
3.2.1.4 additional information the optimum growth medium pH is 7.0 for maximum production of CMCase in WSUCF1, and best carbon and energy source is cellulose, overview Geobacillus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Geobacillus sp. ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Bacillus sp. (in: Bacteria) DUSELR13 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Geobacillus sp. WSUCFI ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Geobacillus sp. ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Bacillus sp. (in: Bacteria) DUSELR13 ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Geobacillus sp. WSUCFI ?
-
 ?
3.2.1.4 additional information the organism possesses carboxymethyl cellulase and cellulase activities, overview Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.4 additional information the organism possesses carboxymethyl cellulase and cellulase activities, overview Geobacillus sp. ?
-
 ?
3.2.1.4 additional information the organism possesses carboxymethyl cellulase and cellulase activities, overview Bacillus sp. (in: Bacteria) DUSELR13 ?
-
 ?
3.2.1.4 additional information the organism possesses carboxymethyl cellulase and cellulase activities, overview Geobacillus sp. WSUCFI ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Carboxymethyl cellulase
-
 Bacillus sp. (in: Bacteria)
3.2.1.4 Carboxymethyl cellulase
-
 Geobacillus sp.
3.2.1.4 CMCase
-
 Bacillus sp. (in: Bacteria)
3.2.1.4 CMCase
-
 Geobacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 70
-
 carboxymethyl cellulose Geobacillus sp.
3.2.1.4 75
-
 carboxymethyl cellulose Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
 CMCase, 1 day, no loss of activity, 46% activity remaning after 26 days, inactivation after 40 days Bacillus sp. (in: Bacteria)
3.2.1.4 50 60 CMCase, 100% activity after 1 day, 80% activity remaining after 40 days Geobacillus sp.
3.2.1.4 60
-
 CMCase, 97% activity remaining after 1 day, below 5% activity remaining after 40 days Bacillus sp. (in: Bacteria)
3.2.1.4 70
-
 CMCase, 78% activity remaining after 1 day Bacillus sp. (in: Bacteria)
3.2.1.4 70
-
 CMCase, 89% activity after 1 day, complete loss of activity after 20 days Geobacillus sp.
3.2.1.4 80
-
 CMCase, 10% activity remaining after 1 day Bacillus sp. (in: Bacteria)
3.2.1.4 80
-
 CMCase, complete loss of activity after 21 days Geobacillus sp.
3.2.1.4 90 100 CMCase, 60 min, complete inactivation Bacillus sp. (in: Bacteria)
3.2.1.4 90 100 CMCase, 60 min, complete inactivation Geobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
 carboxymethyl cellulose Bacillus sp. (in: Bacteria)
3.2.1.4 5
-
 carboxymethyl cellulose Geobacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 11 activity range, carboxymethyl cellulose Bacillus sp. (in: Bacteria)
3.2.1.4 3 11 activity range, carboxymethyl cellulose Geobacillus sp.