Literature summary extracted from

Saarem, K.; Pedersen, J.I.
25-Hydroxylation of 1 alpha-hydroxyvitamin D-3 in rat and human liver (1985), Biochim. Biophys. Acta, 840, 117-126.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.14.14.24	bovine serum albumin	stimulation	Rattus norvegicus
1.14.14.24	additional information	stimulation by addition of cytosolic protein, which remains after boiling the cytosolic protein	Rattus norvegicus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.14.24	0.02	-	vitamin D3	mitochondrial enzyme, pH not specified in the publication, temperature not specified in the publication	Rattus norvegicus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.14.14.24	microsome	-	Rattus norvegicus	-	-
1.14.14.24	mitochondrion	heavy mitochondrial fraction	Rattus norvegicus	5739	-
1.14.14.24	mitochondrion	heavy mitochondrial fraction, highest activity found	Rattus norvegicus	5739	-
1.14.14.24	additional information	no activity in cytosol or nucleus	Rattus norvegicus	-	-
1.14.14.24	nucleus	-	Rattus norvegicus	5634	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.24	Rattus norvegicus	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.14.14.24	liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.24	vitamin D3 + O2 + NADPH + H+	-	Rattus norvegicus	25-hydroxyvitamin D3 + NADP+ + H2O	-	?

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)