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Literature summary extracted from

  • Ohshima, T.; Soda, K.
    Valine dehydrogenase from a non-spore-forming bacterium, Alcaligenes faecalis: purification and characterization (1993), Biochim. Biophys. Acta, 1162, 221-226.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.23 4,4'-Dithiopyridine 0.01 mM, 95% inhibition Alcaligenes faecalis
1.4.1.23 5,5'-dithiobis(2-nitrobenzoic acid) 0.01 mM, 95% inhibition Alcaligenes faecalis
1.4.1.23 CuSO4 1 mM, 13% inhibition Alcaligenes faecalis
1.4.1.23 HgCl2 0.1 mM, 91% inhibition Alcaligenes faecalis
1.4.1.23 additional information not inhibitory at 1 mM: ATP, ADP, AMP, GTP, GDP, MgSO4, MgCl2, ZnCl2, COSO4, BaCl2, EDTA and o-phenanthroline Alcaligenes faecalis
1.4.1.23 N-ethylmaleimide 0.01 mM, 73% inhibition Alcaligenes faecalis
1.4.1.23 p-chloromercuribenzoate 0.001 mM, 95% inhibition Alcaligenes faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.23 40000
-
2 * 40000, SDS-PAGE Alcaligenes faecalis
1.4.1.23 72000
-
gel filtration Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.23 Alcaligenes faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.23 partial Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.23 2-oxobutanoate + NH3 + NADH + H+ 22% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-2-aminobutanoate + H2O + NAD+
-
r
1.4.1.23 2-oxohexanoate + NH3 + NADH + H+ 6.3% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-norleucine + H2O + NAD+
-
r
1.4.1.23 2-oxoisohexanoate + NH3 + NADH + H+ 58% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-leucine + H2O + NAD+
-
r
1.4.1.23 2-oxoisovalerate + NH3 + NADH + H+
-
Alcaligenes faecalis L-valine + H2O + NAD+
-
r
1.4.1.23 2-oxovalerate + H2O + NAD+ 5.0% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-norvaline + NH3 + NADH + H+
-
r
1.4.1.23 3-methyl-2-oxopentanoate + NH3 + NADH + H+ 68% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-isoleucine + H2O + NAD+
-
r
1.4.1.23 L-2-aminobutanoate + H2O + NAD+ 33% of the activity with L-valine Alcaligenes faecalis 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-isoleucine + H2O + NAD+ 72% of the activity with L-valine Alcaligenes faecalis 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-leucine + H2O + NAD+ 73% of the activity with L-valine Alcaligenes faecalis 2-oxoisohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norleucine + H2O + NAD+ 16% of the activity with L-valine Alcaligenes faecalis 2-oxohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norvaline + H2O + NAD+ 44% of the activity with L-valine Alcaligenes faecalis 2-oxovalerate + NH3 + NADH + H+
-
r
1.4.1.23 L-valine + H2O + NAD+
-
Alcaligenes faecalis 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.23 dimer 2 * 40000, SDS-PAGE Alcaligenes faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.23 40
-
stable up to Alcaligenes faecalis
1.4.1.23 45
-
5 min, 50% loss of activity Alcaligenes faecalis
1.4.1.23 50
-
5 min, complete loss of activity Alcaligenes faecalis
1.4.1.23 55
-
5 min, presence of 20% glycerol, retains full activity Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.23 9
-
reductive amination Alcaligenes faecalis
1.4.1.23 10.7
-
oxidative demaination Alcaligenes faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.23 6.5 9.7 stable for at least 5 min Alcaligenes faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.23 3-acetylpyridine-deamino-NAD+ 150% of the activity with NAD+ Alcaligenes faecalis
1.4.1.23 3-acetylpyridine-NAD+ 714% of the activity with NAD+ Alcaligenes faecalis
1.4.1.23 3-pyridinealdehyde-NAD+ 173% of the activity with NAD+ Alcaligenes faecalis
1.4.1.23 deamino-NAD+ 440% of the activity with NAD+ Alcaligenes faecalis
1.4.1.23 additional information no cofactor: NADP+ Alcaligenes faecalis
1.4.1.23 NAD+
-
Alcaligenes faecalis
1.4.1.23 NADH
-
Alcaligenes faecalis