EC Number | Organism | UniProt | Comment | Textmining |
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1.18.1.5 | Pseudomonas putida | - |
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EC Number | Subunits | Comment | Organism |
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1.18.1.5 | More | cross-linking of putidaredoxin and putidaredoxin reductase by 1-ethyl 3-[3-(dimethylamino)propyl]carbodiimide, EDC. EDC promotes formation of stoichiometric Pdr-Pdx complexes only when carboxyl groups on putidaredoxin are activated. The putidaredoxin-putidaredoxin reductase C73S/C85S conjugate protein is more efficient in electron transfer to cytochrome c and, in the presence of saturating levels of P450cam, more effectively supports camphor hydroxylation. The cross-linked complex is physiologically relevant and represents a suitable model for mechanistic studies, and molecular recognition between putidaredoxin and putidaredoxin reductase is redox-controlled and assisted by the putidaredoxin Glu72 and putidaredoxin reducase Lys409 charge-charge interactions | Pseudomonas putida |