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Literature summary extracted from

  • Catucci, G.; Gilardi, G.; Jeuken, L.; Sadeghi, S.
    In vitro drug metabolism by C-terminally truncated human flavin-containing monooxygenase 3 (2012), Biochem. Pharmacol., 83, 551-558.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.148 wild type and truncated enzymes are expressed in Escherichia coli JM109 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.148 0.022
-
 sulindac sulfide truncated recombinant enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
1.14.13.148 0.025
-
 sulindac sulfide wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
1.14.13.148 0.033
-
 Methimazole wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
1.14.13.148 0.035
-
 Methimazole truncated recombinant enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
1.14.13.148 0.045
-
 Benzydamine truncated recombinant enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
1.14.13.148 0.056
-
 Benzydamine wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.148 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.148 60000
-
 x * 60000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2 Homo sapiens
-
 N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.148 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.148 DEAE ion exchange column chromatography and Ni affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.148 benzydamine + NADPH + H+ + O2
-
 Homo sapiens benzydamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 danusertib + NADPH + H+ + O2
-
 Homo sapiens danusertib N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 methimazole + NADPH + H+ + O2
-
 Homo sapiens methimazole N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Homo sapiens N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 sulindac sulfide + NADPH + H+ + O2
-
 Homo sapiens sulindac + NADP+ + H2O
-
 ?
1.14.13.148 tozasertib + NADPH + H+ + O2
-
 Homo sapiens tozasertib N-oxide + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.148 ? x * 60000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.13.148 flavin-containing monooxygenase 3
-
 Homo sapiens
1.14.13.148 FMO3
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.148 FAD
-
 Homo sapiens
1.14.13.148 NADPH required for activity Homo sapiens