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Literature summary extracted from

  • Lack, N.; Kawamura, A.; Fullam, E.; Laurieri, N.; Beard, S.; Russell, A.; Evangelopoulos, D.; Westwood, I.; Sim, E.
    Temperature stability of proteins essential for the intracellular survival of Mycobacterium tuberculosis (2009), Biochem. J., 418, 369-378.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.17 expressed in Pseudomonas putida strain KT2442 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.17 0.23
-
 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.7.1.17 cytosol
-
 Mycobacterium tuberculosis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.17 33800
-
 x * 33800, His6-tagged enzyme, estimated from SDS-PAGE Mycobacterium tuberculosis
3.7.1.17 132000
-
 calculated from amino acid sequence Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.17 Mycobacterium tuberculosis
-
-
-
3.7.1.17 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.17 Ni-NTA column chromatography, gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.17 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
3.7.1.17 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.17 homotetramer x * 33800, His6-tagged enzyme, estimated from SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.7.1.17 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase
-
 Mycobacterium tuberculosis
3.7.1.17 HsaD
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.7.1.17 60 70 the enzyme retains more than 95% of its activity after incubation at 60°C for 30 min. Hydrolytic activity is significantly decreased after incubation at 70°C Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.17 0.25
-
 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.7.1.17 1.129
-
 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid apparent value, in 100 mM of phosphate buffer (pH 7.4) with 20 mM NaCl, temperature not specified in the publication Mycobacterium tuberculosis