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Literature summary extracted from

  • Shinkyo, R.; Sakaki, T.; Kamakura, M.; Ohta, M.; Inouye, K.
    Metabolism of vitamin D by human microsomal CYP2R1 (2004), Biochem. Biophys. Res. Commun., 324, 451-457.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.24 expression in Saccharomyces cerevisiae Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.24 0.00045
-
 vitamin D2 pH 7.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.24 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.24 Iron addition of NAD+ in presence of 1alpha-hydroxy-vitamin D2 to CYP2R1 induces a typical type I spectrum, indicating a change of the heme iron of CYP2R1 from a low-spin state to a high-spin state Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.24 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.24 1alpha-hydroxy-vitamin D2 + O2 + [reduced NADPH-hemoprotein reductase] + H+
-
 Homo sapiens 1alpha,25-dihydroxy-vitamin D2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.24 1alpha-hydroxy-vitamin D3 + O2 + [reduced NADPH-hemoprotein reductase] + H+
-
 Homo sapiens 1alpha,25-dihydroxy-vitamin D3 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.24 additional information CYP2R1 shows no detectable 1alpha-hydroxylation activity Homo sapiens ?
-
 ?
1.14.14.24 vitamin D2 + O2 + NADPH + H+
-
 Homo sapiens 25-hydroxy-vitamin D2 + NADP+ + H2O
-
 ?
1.14.14.24 vitamin D3 + O2 + NADPH + H+
-
 Homo sapiens 25-hydroxy-vitamin D3 + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.24 CYP2R1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.24 0.017
-
 vitamin D2 pH 7.4, 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.24 heme addition of NAD+ in presence of 1alpha-hydroxy-vitamin D2 to CYP2R1 induces a typical type I spectrum, indicating a change of the heme iron of CYP2R1 from a low-spin state to a high-spin state Homo sapiens