EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.24 | expression in Escherichia coli | Sus scrofa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.24 | A241G/L243V/F244L/P245R/R246F | mutation in substrate recognition site 3, to the equivalent residues in CYP2D6, an enzyme not active in 25-hydroxylation. The 25-hydroxylase activity of the mutant is completely lost whereas the activity toward tolterodine remains virtually unaffected | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.24 | 0.001 | - |
bufuralol | pH 7.4, 37°C, mutant A241G/L243V/F244L/P245R/R246F | Sus scrofa | |
1.14.14.24 | 0.0014 | - |
bufuralol | pH 7.4, 37°C, wild-type | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.24 | microsome | - |
Sus scrofa | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.24 | Sus scrofa | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.24 | 1alpha-hydroxyvitamin D3 + O2 + NADPH + H+ | - |
Sus scrofa | 1alpha,25-dihydroxy-vitamin D3 + NADP+ + H2O | - |
? | |
1.14.14.24 | bufuralol + O2 + NADPH + H+ | - |
Sus scrofa | 1'-hydroxybufuralol + NADP+ + H2O | - |
? | |
1.14.14.24 | tolterodine + O2 + NADPH + H+ | - |
Sus scrofa | ? + NADP+ + H2O | - |
? | |
1.14.14.24 | vitamin D3 + O2 + NADPH + H+ | - |
Sus scrofa | 25-hydroxyvitamin D3 + NADP+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.24 | CYP2D25 | - |
Sus scrofa |