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Literature summary extracted from

  • Jones, C.G.; Keeling, C.I.; Ghisalberti, E.L.; Barbour, E.L.; Plummer, J.A.; Bohlmann, J.
    Isolation of cDNAs and functional characterisation of two multi-product terpene synthase enzymes from sandalwood, Santalum album L (2008), Arch. Biochem. Biophys., 477, 121-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.112 expression in Escherichia coli Santalum album
4.2.3.126 SasesquiTPS1, DNA and amino acid sequence determination and analysis, sequence comparison, functional expression of His6-tagged full-length enzyme in Escherichia coli Santalum album

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.112 Mg2+ or Mn2+, required Santalum album
4.2.3.112 Mn2+ or Mg2+, required Santalum album
4.2.3.126 Mg2+ activates, required Santalum album
4.2.3.126 Mn2+ activates, 10fold less affective compared to Mg2+ Santalum album

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.112 66000
-
 x * 66000, SDS-PAGE Santalum album

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.112 Santalum album
-
-
-
4.2.3.126 Santalum album B5A435
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.126 recombinant HIs6-tagged SasesquiTPS1 from Escherichia coli by nickel affinity chromatography Santalum album

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.112 geranyl diphosphate
-
 Santalum album (+)-alpha-terpineol + diphosphate products are 44% (+)-alpha-terpineol and 33.6% (-)-limonene, along with small quantities of linalool, myrcene, alpha-pinene, (+)-sabinene and geraniol ?
4.2.3.112 additional information enzyme additionally produces beta-bisabolene from (E,E)-farnesyl diphosphate, with low efficiency Santalum album ?
-
 ?
4.2.3.126 (2E,6E)-farnesyl diphosphate
-
 Santalum album gamma-muurolene + diphosphate SasesquiTPS1 with (2E,6E)-farnesyl diphosphate as substrate produces a complex mixture of mono- and bicyclic sesquiterpenes. The main products as analysed by GC-MS and GC-FID are the cyclic alcohol germacrene D-4-ol, and helminthogermacrene. Also present are alpha-bulnesene, gamma-muurolene, alpha- and beta-selinenes, as well as several other minor bicyclic compounds, alpha-humulene and nerolidol are detected in proportions below 5% ?

Subunits

EC Number Subunits Comment Organism
4.2.3.112 ? x * 66000, SDS-PAGE Santalum album
4.2.3.126 More SasesquiTPS1 shows the R(R/P)X8W motif at positions 19-29, and the DDXXD motif Santalum album

Synonyms

EC Number Synonyms Comment Organism
4.2.3.112 monoTPS1
-
 Santalum album
4.2.3.126 SasesquiTPS1
-
 Santalum album
4.2.3.126 sesquiterpene synthase
-
 Santalum album