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Literature summary extracted from

  • Phillips, M.A.; Wildung, M.R.; Williams, D.C.; Hyatt, D.C.; Croteau, R.
    cDNA isolation, functional expression, and characterization of (+)-alpha-pinene synthase and (-)-alpha-pinene synthase from loblolly pine (Pinus taeda): stereocontrol in pinene biosynthesis (2003), Arch. Biochem. Biophys., 411, 267-276.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.119 expression in Escherichia coli Pinus taeda
4.2.3.121 expression in Escherichia coli Pinus taeda

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.121 modeling of structure against the tobacco sesquiterpene synthase 5-epi-aristolochene synthase and the monoterpene synthase bornyl diphosphate synthase from common sage Pinus taeda

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.119 0.0014
-
 geranyl diphosphate pH 6.5, 31°C Pinus taeda
4.2.3.121 0.047
-
 geranyl diphosphate pH 6.5, 31°C Pinus taeda

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.119 K+ required for optimum activity, NH4+ may substitute Pinus taeda
4.2.3.119 Mg2+ or Mn2+, required. 67% of reaction velocity with Mn2+ Pinus taeda
4.2.3.119 Mn2+ or Mg2+, required. Mn2+ is preferred Pinus taeda
4.2.3.119 NH4+ may substitute for K+ for optimum activity Pinus taeda
4.2.3.121 K+ required for optimum activity, NH4+ may substitute Pinus taeda
4.2.3.121 Mg2+ or Mn2+, required. 67% of reaction velocity with Mn2+ Pinus taeda
4.2.3.121 Mn2+ or Mg2+, required. Mn2+ is preferred Pinus taeda
4.2.3.121 NH4+ may substitute for K+ for optimum activity Pinus taeda

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.119 64500
-
 x * 64500, SDS-PAGE of recombinant protein lacking transit peptide Pinus taeda
4.2.3.121 64500
-
 x * 64500, SDS-PAGE of recombinant protein lacking transit peptide Pinus taeda

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.119 Pinus taeda Q84KL6
-
-
4.2.3.121 Pinus taeda Q84KL3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.119 proteolytic modification sequence contains a putative transit peptide Pinus taeda
4.2.3.121 proteolytic modification sequence contains a putative transit peptide Pinus taeda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.119 geranyl diphosphate
-
 Pinus taeda (-)-alpha-pinene + diphosphate products are 79% (-)-alpha-pinene, 4.25 (-)-beta-pinene, almost racemic mixtures of camphene and limonene and small amounts of (+)-alpha-pinene and (+)-beta-pinene ?
4.2.3.121 geranyl diphosphate
-
 Pinus taeda (+)-alpha-pinene + diphosphate products are 97% (+)-alpha-pinene, 3% (-)-alpha-pinene ?

Subunits

EC Number Subunits Comment Organism
4.2.3.119 ? x * 64500, SDS-PAGE of recombinant protein lacking transit peptide Pinus taeda
4.2.3.121 ? x * 64500, SDS-PAGE of recombinant protein lacking transit peptide Pinus taeda

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.119 7
-
-
 Pinus taeda
4.2.3.121 7.5
-
-
 Pinus taeda

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.119 6.2
-
 half-maximal reaction velocity Pinus taeda
4.2.3.119 7.8
-
 half-maximal reaction velocity Pinus taeda
4.2.3.121 6.5
-
 half-maximal reaction velocity Pinus taeda
4.2.3.121 7.5
-
 half-maximal reaction velocity Pinus taeda