EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.153 | FAD | exogenous, activates the enzyme slightly | Salvia officinalis | |
1.14.13.153 | FMN | exogenous, activates the enzyme | Salvia officinalis | |
1.14.13.153 | octyl beta-D-glucoside | 35% activation at 0.12% detergent, complete inhibition at 0.48% detergent | Salvia officinalis | |
1.14.13.153 | sodium cholate | 40% activation at 0.30% detergent, 17% inhibition at 0.48% detergent | Salvia officinalis | |
1.14.13.153 | Zwittergent 3-08 | 12% activation at 0.24% detergent | Salvia officinalis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.153 | CO | photoreversible inhibition | Salvia officinalis | |
1.14.13.153 | NADP+ | NADP+ competitively inhibits P-450-dependent reactions, 40-45% inhibition of hydroxylation at 2 mM | Salvia officinalis | |
1.14.13.153 | octyl beta-D-glucoside | 35% activation at 0.12% detergent, complete inhibition at 0.48% detergent | Salvia officinalis | |
1.14.13.153 | sodium cholate | 40% activation at 0.30% detergent, 17% inhibition at 0.48% detergent | Salvia officinalis | |
1.14.13.153 | sodium deoxycholate | 80% inhibition at 0.06% detergent | Salvia officinalis | |
1.14.13.153 | sodium taurocholate | 13% inhibition at 0.30% detergent | Salvia officinalis | |
1.14.13.153 | sodium taurodeoxycholate | 91% inhibition at 0.06% detergent | Salvia officinalis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.13.153 | microsome | localization of sabinene hydroxylase in sage leaf homogenates by differential centrifugation indicated that activity is restricted to light membranes | Salvia officinalis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.153 | additional information | no effect on enzyme activity by 1-10 mM of Mg2+, Mn2+, Ca2+, Cu2+, and Fe2+ | Salvia officinalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.153 | (+)-sabinene + NADPH + H+ + O2 | Salvia officinalis | - |
(+)-cis-sabinol + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.153 | Salvia officinalis | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.153 | epidermis | - |
Salvia officinalis | - |
1.14.13.153 | leaf | - |
Salvia officinalis | - |
1.14.13.153 | seedling | etiolated | Salvia officinalis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.153 | (+)-sabinene + NADPH + H+ + O2 | - |
Salvia officinalis | (+)-cis-sabinol + NADP+ + H2O | - |
? | |
1.14.13.153 | (+)-sabinene + NADPH + H+ + O2 | the hydroxylase is highly specific for (+)-sabinene as substrate. Addition of sabinene to solubilized microsomes provides a typical type I binding spectrum | Salvia officinalis | (+)-cis-sabinol + NADP+ + H2O | GC product analysis | ? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.153 | 20 | - |
assay at | Salvia officinalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.153 | 7.4 | - |
- |
Salvia officinalis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.153 | 6.8 | 8.6 | 50% of maximal activity at pH 6.8 and pH 8.6 | Salvia officinalis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.153 | NADPH | - |
Salvia officinalis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.153 | evolution | the enzyme is a cytochrome P-450-dependent mixed function oxygenase and belongs to the cytochrome P450 oxygenase family | Salvia officinalis |
1.14.13.153 | metabolism | the enzyme catalyzes the synthesis of (+)-cis-sabinol, which is a key step in the biosynthesis of C3-oxygenated thujane monoterpenes, overview | Salvia officinalis |
1.14.13.153 | additional information | (+)-sabinene is a major olefinic constituent of the volatile oil of immature Salvia oficinalis | Salvia officinalis |