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Literature summary extracted from

  • Poulose, A.; Croteau, R.
    gamma-Terpinene synthetase: A key enzyme in the biosynthesis of aromatic monoterpenes (1978), Arch. Biochem. Biophys., 191, 400-411.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.3.114 addition of either 20% glycerol or 1 mM substrate does not significantly improve stability Thymus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.114 EDTA 1 mM, complete inactivation Thymus vulgaris
4.2.3.114 additional information not inhibitory: gamma-terpinene, p-cymene, thymol Thymus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.114 0.009
-
 neryl diphosphate pH 6.6, 30°C Thymus vulgaris
4.2.3.114 0.014
-
 geranyl diphosphate pH 6.6, 30°C Thymus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.114 Mg2+ or Mn2+, required, Mg2+ is preferred over Mn2+ Thymus vulgaris
4.2.3.114 Mn2+ or Mg2+, required, about 60% of the efficiency qith Mg2+ Thymus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.114 96000
-
 gel filtration Thymus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.114 Thymus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.114 partial Thymus vulgaris

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.114 geranyl diphosphate = gamma-terpinene + diphosphate mechanism involves the cyclizazion of the acyclic precursor, loss of a proton from C5 to form the delta4 double bond, and a 1,2-hydride shift fom C4 to C8 to give gamma-terpinene Thymus vulgaris
a

Storage Stability

EC Number Storage Stability Organism
4.2.3.114 0°C, 40 mM sodium phosphate, 5 mM MgCl2, 0.5 mM dithioerythritol, loss of 60% of activity within 1 week Thymus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.114 geranyl diphosphate
-
 Thymus vulgaris gamma-terpinene + diphosphate
-
 ?
4.2.3.114 neryl diphosphate
-
 Thymus vulgaris gamma-terpinene + diphosphate 75% gamma-terpinene, 20% alpha-thujene, 5% alpha-terpineol, partially purified enzyme ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.114 6.8
-
-
 Thymus vulgaris