EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.3 | additional information | construction of a series of hybrid enzymes by interchanging domains of glucoamylase Sta1 from Saccharomyces cerevisiae and beta-glucosidase Bgl1 from Saccharomycopsis fibuligera strain ATCC 9947 based on the homology-based structural models of the two proteins. The replacement of native Bgl1 signal peptide by that of Sta1, SPS-Bgl1, increases the production of the enzyme by about threefold without affecting the ratio between the values of activity associated to cells and free in the medium | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Saccharomyces cerevisiae | - |
var. diastaticus | - |
3.2.1.3 | Saccharomyces cerevisiae BY4741 | - |
var. diastaticus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | soluble starch + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
3.2.1.3 | soluble starch + H2O | - |
Saccharomyces cerevisiae BY4741 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.3 | More | glucoamylase is a two-domain protein composed by a N-terminal serinethreonine-rich domain and a C-terminal domain with the typical structure of the catalytic domain of fungal glucoamylases | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.3 | glucoamylase | - |
Saccharomyces cerevisiae |
3.2.1.3 | Sta1 | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 37 | - |
assay at | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 5 | - |
assay at | Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.3 | additional information | the replacement of native beta-glucosidase Bgl1 signal peptide by that of Sta1, SPS-Bgl1, increases the production of the enzyme by about threefold without affecting the ratio between the values of activity associated to cells and free in the medium | Saccharomyces cerevisiae |