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Literature summary extracted from

  • Braun, S.D.; Hofmann, J.; Wensing, A.; Ullrich, M.S.; Weingart, H.; Voelksch, B.; Spiteller, D.
    Identification of the biosynthetic gene cluster for 3-methylarginine, a toxin produced by Pseudomonas syringae pv. syringae 22d/93 (2010), Appl. Environ. Microbiol., 76, 2500-2508.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.243 gene mrsA, DNA and amino acid sequence determination and analysis, sequences of genes mrsA and mrsB reveal an overlapping region in the start/stop sequences, sequence comparisons, mrsA-ATGA-mrsB, overexpression of the His-tagged MrsA in Escherichia coli strain BL21(DE3) Pseudomonas syringae

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.243 additional information Tn5 transposon mutagenesis Pseudomonas syringae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.243 S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate Pseudomonas syringae
-
 S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.243 Pseudomonas syringae D5FKJ3 pv. syringae 22d/93
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.243 recombinant His-tagged MrsA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas syringae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.243 S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate
-
 Pseudomonas syringae S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate
-
 ?
2.1.1.243 S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate
-
 Pseudomonas syringae S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate NMR and mass spectrometric product analysis ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.243 mrsA
-
 Pseudomonas syringae
2.1.1.243 S-adenosylmethionine-dependent methyltransferase
-
 Pseudomonas syringae
2.1.1.243 SAM-dependent methyltransferase
-
 Pseudomonas syringae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.243 28
-
 assay at Pseudomonas syringae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.243 8.5
-
 assay at Pseudomonas syringae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.243 S-adenosyl-L-methionine dependent on Pseudomonas syringae

General Information

EC Number General Information Comment Organism
2.1.1.243 metabolism the enzyme is involved inthe biosynthesis of the rare amino acid 3-methylarginine Pseudomonas syringae
2.1.1.243 physiological function the enzyme is involved inthe biosynthesis of the rare amino acid 3-methylarginine, which is highly active against the closely related soybean pathogen Pseudomonas syringae pv. glycinea. Since these pathogens compete for the same habitat, Pss22d is a promising candidate for biocontrol of P. syringae pv. glycinea Pseudomonas syringae