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Literature summary extracted from

  • Singh, A.K.; Chhatpar, H.S.
    Purification and characterization of chitinase from Paenibacillus sp. D1 (2011), Appl. Biochem. Biotechnol., 164, 77-88.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.14 the enzyme exhibits higher stability in presence of commonly used protectant fungicides Captan, Carbendazim, and Mancozeb compared to control Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information determination of kinetic constants and thermodynamic parameters of the enzyme Paenibacillus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Paenibacillus sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 56560
-
 x * 56560, SDS-PAGE Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Paenibacillus sp.
-
-
-
3.2.1.14 Paenibacillus sp. D1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme, 52.3fold by ammonium sulfate fractionation and ion exchange chromatography Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 492.4
-
 purified enzyme, pH 5.0, 50°C Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O the enzyme shows a high affinity of the enzyme for chitin Paenibacillus sp. ?
-
 ?
3.2.1.14 chitin + H2O the enzyme shows a high affinity of the enzyme for chitin Paenibacillus sp. D1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 56560, SDS-PAGE Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Paenibacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 25 60 activity range Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40
-
 purified enzyme, half-life is 370.68 min Paenibacillus sp.
3.2.1.14 50
-
 purified enzyme, half-life is 255.15 min Paenibacillus sp.
3.2.1.14 80
-
 purified enzyme, half-life is 22.61 min Paenibacillus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 83.46
-
 chitin pH 5.0, 50°C Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Paenibacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 10 85.63% of maximal activity at pH 4.0, maximal activity at pH 5.0, and 90.61% of maximal activity pH 8.0 Paenibacillus sp.

General Information

EC Number General Information Comment Organism
3.2.1.14 physiological function chitinases are glycoside hydrolases that catalyze the degradation of chitin, a linear beta-1,4-linked polymer of N-acetylglucosamine Paenibacillus sp.