EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.22.37 | biotechnology | a naive camel nanobody library is constructed and phage display is used to select one nanobody toward RgpB with picomolar affinity. The nanobody is highly specific for RgpB given that it does not bind to the homologous gingipain HRgpA, indicating the presence of a binding epitope within the immunoglobulin-like domain of RgpB. RgpB can be used as a specific biomarker for Porphyromonas gingivalis infection | Porphyromonas gingivalis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.37 | RgpB with the C-terminal His-tag is produced by the Porphyromonas gingivalis W83 strain bearing the modified rgpB gene | Porphyromonas gingivalis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.37 | Porphyromonas gingivalis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.37 | using affinity and ion exchange chromatography | Porphyromonas gingivalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.37 | Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | Nalpha-benzoyl-L-Arg + 4-nitroaniline | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.37 | RgpB | - |
Porphyromonas gingivalis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.37 | 22 | - |
assay at | Porphyromonas gingivalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.37 | 7.6 | - |
assay at | Porphyromonas gingivalis |