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Literature summary extracted from

  • Skottrup, P.D.; Leonard, P.; Kaczmarek, J.Z.; Veillard, F.; Enghild, J.J.; OKennedy, R.; Sroka, A.; Clausen, R.P.; Potempa, J.; Riise, E.
    Diagnostic evaluation of a nanobody with picomolar affinity toward the protease RgpB from Porphyromonas gingivalis (2011), Anal. Biochem., 415, 158-167.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.22.37 biotechnology a naive camel nanobody library is constructed and phage display is used to select one nanobody toward RgpB with picomolar affinity. The nanobody is highly specific for RgpB given that it does not bind to the homologous gingipain HRgpA, indicating the presence of a binding epitope within the immunoglobulin-like domain of RgpB. RgpB can be used as a specific biomarker for Porphyromonas gingivalis infection Porphyromonas gingivalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.37 RgpB with the C-terminal His-tag is produced by the Porphyromonas gingivalis W83 strain bearing the modified rgpB gene Porphyromonas gingivalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.37 using affinity and ion exchange chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.37 RgpB
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.37 22
-
 assay at Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.37 7.6
-
 assay at Porphyromonas gingivalis