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Literature summary extracted from

  • Wickham, S.; West, M.; Cook, P.; Hanigan, M.
    Gamma-glutamyl compounds: Substrate specificity of gamma-glutamyl transpeptidase enzymes (2011), Anal. Biochem., 414, 208-214.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 expressed in Pichia pastoris Homo sapiens
3.4.19.14 expressed in Pichia pastoris Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 serine-borate competitive inhibitor which is 8fold more potent in inhibiting isoform GGT1 than in inhibiting isoform GGT5; competitive inhibitor which is 8fold more potent in inhibiting isoform GGT1 than in inhibiting isoform GGT5 Homo sapiens
3.4.19.13 serine borate competitive, 8fold less effective as an inhibitor of isoform GGT5 than of GGT1; competitive, 8fold less effective as an inhibitor of isoform GGT5 than of GGT1 Homo sapiens
3.4.19.14 serine-borate 8fold more potent in inhibiting GGT1 than in inhibiting GGT5 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.008
-
 GSSG isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0099
-
 S-Methylglutathione isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0102
-
 leukotriene C4 isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0105
-
 GSH isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0106
-
 GSH isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0108
-
 leukotriene C4 isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0131
-
 S-(4-nitro-benzyl)glutathione isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0148
-
 S-(4-nitro-benzyl)glutathione isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0182
-
 S-Methylglutathione isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0334
-
 5-L-glutamyl-L-leucine isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0346
-
 glutathione sulfonic acid isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0426
-
 GSSG isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 0.0751
-
 glutathionesulfonic acid isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
3.4.19.13 0.0088
-
 oxidized glutathione pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0102
-
 leukotriene C4 pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0105
-
 reduced glutathione pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0106
-
 reduced glutathione pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0108
-
 leukotriene C4 pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0131
-
 S-(4-nitro-benzyl)glutathione pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0148
-
 S-(4-nitro-benzyl)glutathione pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0182
-
 S-Methylglutathione pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0334
-
 gamma-glutamyl L-leucine pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0346
-
 glutathione sulfonic acid pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0426
-
 oxidized glutathione pH 7.4, 37°C Homo sapiens
3.4.19.13 0.0751
-
 glutathione sulfonic acid pH 7.4, 37°C Homo sapiens
3.4.19.13 0.099
-
 S-Methylglutathione pH 7.4, 37°C Homo sapiens
3.4.19.14 0.0102
-
 leukotriene C4 in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
3.4.19.14 0.0148
-
 S-(4-nitro-benzyl)glutathione in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
3.4.19.14 0.0182
-
 S-Methylglutathione in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
3.4.19.14 0.0751
-
 glutathione sulfonic acid in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
3.4.19.14 0.105
-
 reduced glutathione in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
3.4.19.14 0.426
-
 oxidized glutathione in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 22000
-
 1 * 64000 + 1 * 22000, isoform GGT1, SDS-PAGE Homo sapiens
2.3.2.2 25000
-
 1 * 64000 + 1 * 25000, isoform GGT5, SDS-PAGE Homo sapiens
2.3.2.2 64000
-
 1 * 64000 + 1 * 22000, isoform GGT1, SDS-PAGE Homo sapiens
2.3.2.2 64000
-
 1 * 64000 + 1 * 25000, isoform GGT5, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Homo sapiens
-
-
-
2.3.2.2 Homo sapiens P19440 isoform GGT1
-
3.4.19.13 Homo sapiens P19440
-
-
3.4.19.13 Homo sapiens P36269
-
-
3.4.19.14 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine substrate for isoform GGT1 Homo sapiens 5-L-glutamyl-glycylglycine + 4-nitroaniline
-
 ?
2.3.2.2 5-L-glutamyl-L-leucine + glycylglycine
-
 Homo sapiens L-Leu + 5-L-glutamyl-glycylglycine
-
 ?
2.3.2.2 glutathione sulfonic acid + glycylglycine
-
 Homo sapiens ?
-
 ?
2.3.2.2 glutathionesulfonic acid + glycylglycine
-
 Homo sapiens ?
-
 ?
2.3.2.2 GSH + glycylglycine
-
 Homo sapiens L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
 ?
2.3.2.2 GSSG + glycylglycine
-
 Homo sapiens L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
 ?
2.3.2.2 leukotriene C4 + glycylglycine
-
 Homo sapiens leukotriene D4 + 5-L-glutamyl-glycylglycine
-
 ?
2.3.2.2 additional information isoform GGT5 does not cleave 5-L-glutamyl-4-nitroanilide and gamma-glutamyl leucine Homo sapiens ?
-
 ?
2.3.2.2 S-(4-nitro-benzyl)glutathione + glycylglycine
-
 Homo sapiens ?
-
 ?
2.3.2.2 S-methylglutathione + glycylglycine
-
 Homo sapiens ?
-
 ?
3.4.19.13 gamma-glutamyl L-leucine + H2O
-
 Homo sapiens L-leucine + L-glutamate
-
 ?
3.4.19.13 glutathione sulfonic acid + H2O
-
 Homo sapiens ? + L-glutamate
-
 ?
3.4.19.13 leukotriene C4 + H2O
-
 Homo sapiens ?
-
 ?
3.4.19.13 additional information no substrate: gamma-glutamyl L-leucine Homo sapiens ?
-
 ?
3.4.19.13 additional information protein additionally has EC 2.3.2.2, gamma-glutamyltransferase activity Homo sapiens ?
-
 ?
3.4.19.13 oxidized glutathione + H2O
-
 Homo sapiens ? + L-glutamate
-
 ?
3.4.19.13 reduced glutathione + H2O
-
 Homo sapiens L-cysteinylglycine + L-glutamate
-
 ?
3.4.19.13 S-(4-nitro-benzyl)glutathione + H2O
-
 Homo sapiens S-(4-nitro-benzyl)-L-cysteinylglycine + L-glutamate
-
 ?
3.4.19.13 S-methylglutathione + H2O
-
 Homo sapiens S-methyl-L-cysteinylglycine + L-glutamate
-
 ?
3.4.19.14 glutathione sulfonic acid + H2O + glycylglycine
-
 Homo sapiens ? + gamma-glutamyl-glycylglycine
-
 ?
3.4.19.14 leukotriene C4 + H2O
-
 Homo sapiens leukotriene D4 + L-glutamate
-
 ?
3.4.19.14 additional information no activity with gamma-glutamyl leucine Homo sapiens ?
-
 ?
3.4.19.14 oxidized glutathione + H2O + glycylglycine
-
 Homo sapiens ? + gamma-glutamyl-glycylglycine
-
 ?
3.4.19.14 reduced glutathione + H2O + glycylglycine
-
 Homo sapiens ? + gamma-glutamyl-glycylglycine
-
 ?
3.4.19.14 S-(4-nitro-benzyl)glutathione + H2O + glycylglycine
-
 Homo sapiens ? + gamma-glutamyl-glycylglycine
-
 ?
3.4.19.14 S-methylglutathione + H2O + glycylglycine
-
 Homo sapiens ? + gamma-glutamyl-glycylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 heterodimer 1 * 64000 + 1 * 22000, isoform GGT1, SDS-PAGE Homo sapiens
2.3.2.2 heterodimer 1 * 64000 + 1 * 25000, isoform GGT5, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 gamma-glutamyl transpeptidase
-
 Homo sapiens
2.3.2.2 GGT1 isoform Homo sapiens
2.3.2.2 GGT5 isoform Homo sapiens
3.4.19.13 gamma-glutamyl transpeptidase
-
 Homo sapiens
3.4.19.13 GGT1
-
 Homo sapiens
3.4.19.13 GGT5
-
 Homo sapiens
3.4.19.14 gamma-glutamyl leukotrienase
-
 Homo sapiens
3.4.19.14 GGT5 isoform B Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.2 4.2
-
 serine-borate isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
2.3.2.2 41.6
-
 serine-borate isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
3.4.19.13 4.2
-
 serine borate pH 7.4, 37°C Homo sapiens