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Literature summary extracted from

  • Ongeri, E.M.; Anyanwu, O.; Reeves, W.B.; Bond, J.S.
    Villin and actin in the mouse kidney brush-border membrane bind to and are degraded by meprins, an interaction that contributes to injury in ischemia-reperfusion (2011), Am. J. Physiol. Renal Physiol., 301, F871-F882.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.63 recombinantly expressed Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.63 0.00073
-
 actin pH 7.5, 37°C Rattus norvegicus
3.4.24.63 0.00117
-
 villin pH 7.5, 37°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.63 Mus musculus
-
-
-
3.4.24.63 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.63 purified from stably transfected human embryonic kidney HEK-293 cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.63 kidney
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.63 actin + H2O villin and actin bind to the cytoplasmic tail of meprin beta Mus musculus ?
-
 ?
3.4.24.63 actin + H2O villin and actin bind to the cytoplasmic tail of meprin beta Rattus norvegicus ?
-
 ?
3.4.24.63 villin + H2O villin and actin bind to the cytoplasmic tail of meprin beta Mus musculus ?
-
 ?
3.4.24.63 villin + H2O villin and actin bind to the cytoplasmic tail of meprin beta Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.63 Meprin b
-
 Mus musculus
3.4.24.63 Meprin b
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.63 37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.63 0.625
-
 actin pH 7.5, 37°C Rattus norvegicus
3.4.24.63 150
-
 villin pH 7.5, 37°C Rattus norvegicus

General Information

EC Number General Information Comment Organism
3.4.24.63 malfunction following ischemia-reperfusion, meprins and villin redistribute from the brush-border membranes to the cytosol. A 37-kDa actin fragment is detected in protein fractions from wild-type, but not in comparable preparations from meprin knockout mice Rattus norvegicus
3.4.24.63 malfunction following ischemia-reperfusion, meprins and villin redistribute from the brush-border membranes to the cytosol. A 37-kDa actin fragment is detected in protein fractions from wildtype, but not in comparable preparations from meprin knockout mice Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.63 860
-
 actin pH 7.5, 37°C Rattus norvegicus
3.4.24.63 128200
-
 villin pH 7.5, 37°C Rattus norvegicus